Document 0871
 DOCN  M9480871
 TI    Induction of immune mediators by defined lipid fractions from
       Mycobacterium avium serovar 8.
 DT    9410
 AU    Barrow W; Davis T; Wright E; Labrousse V; Bachelet M; Rastogi N;
       Institut Pasteur, Paris, France.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:190 (abstract no. U-102).
       Unique Identifier : AIDSLINE ASM94/94313074
 AB    Because of increasing evidence that M. avium lipids can modulate host
       responses, lipid fractions from M. avium serovar 8 were evaluated for
       their ability to modulate the immune function of human peripheral blood
       mononuclear cells. A total lipid fraction was obtained by the Folch
       extraction procedure and processed by column chromatography to derive
       individual fractions which were defined by the presence of
       glycopeptidolipids (GPL) or related components, and the amount of total
       carbohydrate and 6-deoxyhexose, as determined by the Dubois and Dische
       method, respectively. Total lipid and lipid fractions were assessed for
       their ability to induce prostaglandin E2 (PGE2) and tumor necrosis
       factor-alpha (TNF-alpha) release and their ability to affect macrophage
       function as determined by intracellular growth experiments using an
       unrelated strain of M. avium. All fractions affected human monocyte
       function, with the more apolar fractions having the most adverse effect.
       Only the total lipid and GPL fractions were able to induce significant
       release of TNF-alpha and PGE2. Effectiveness for lipid fractions to be
       immunomodulatory appeared to be dependent upon the amount of
       carbohydrate, particularly with regard to 6-deoxy-hexose content,
       suggesting the importance of GPL and related components in the immune
       response to M. avium.
 DE    Dinoprostone/BIOSYNTHESIS  Human  Lipids/*IMMUNOLOGY/ISOLATION & PURIF
       Lymphocytes/*IMMUNOLOGY  Macrophages/*IMMUNOLOGY/MICROBIOLOGY
       Mycobacterium avium/CLASSIFICATION/GROWTH & DEVELOPMENT/  *IMMUNOLOGY
       Serotyping  Tumor Necrosis Factor/BIOSYNTHESIS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).