Document 0094
 DOCN  M9490094
 TI    Histoincompatibility-associated differences in the phenotypes of murine
       cardiac allograft infiltrating T cells.
 DT    9411
 AU    Carlquist JF; Shelby J; Hammond EH; Greenwood JH; Anderson JL;
       Department of Medicine, University of Utah School of Medicine,; Salt
       Lake City.
 SO    Immunology. 1994 May;82(1):149-53. Unique Identifier : AIDSLINE
       MED/94320992
 AB    Mechanisms of graft rejection may be governed in part by the kind and
       degree of histocompatibility differences between donor and recipient.
       Cardiac allograft rejection was studied in three murine models selected
       to provide disparity at different major histocompatibility complex
       (MHC), minor lymphocyte stimulating (Mls) and other minor
       histocompatibility loci. Graft survival for the A.TL to A.TH combination
       (M3) was significantly longer (median day of rejection 15.0 days) than
       both the B10.A to AKR (M2) or the C57BL/6 to C3H/HeN (M1)
       donor-recipient combinations (median days of rejection: 9.0 days and 9.0
       days respectively; P < 0.001). The infiltration of grafts by T cells was
       examined by removal of grafts serially post-transplantation and
       culturing mechanically disrupted graft tissue with interleukin-2 (IL-2).
       Recovery of T cells by this method revealed highly reproducible
       characteristics (kinetic and phenotypic), unique to each donor-recipient
       combination. Cultures from M1 and M2 grafts had differing CD4/CD8 T-cell
       ratios at days 2 (1.8 versus 0.7, respectively) and 4 (1.6 versus 0.1,
       respectively) post-transplantation. The M3 model differed from M2 (at
       days 4, 8 and 10) and from M1 (at days 8 and 10). At these times,
       cultures of M3 grafts contained a significantly increased percentage of
       CD4 cells and significantly decreased percentage of CD8 cells (CD4/CD8
       ratios 0.9-1.3) by comparison with M1 (CD4/CD8 ratios 0.02-0.04) and M2
       (CD4/CD8 ratios 0.1-0.02). Long-surviving M3 grafts (greater than 30
       days post-transplantation) were compared with grafts removed immediately
       upon cessation of graft function (days 14, 15 and 18
       post-transplantation). There was a significant difference between these
       groups in the ratios of CD4/CD8 T-cell ratios (1.1 versus 0.4,
       respectively). This study suggests that the cellular rejection mechanism
       of a graft is a variable process driven by the individual
       histocompatibility antigen disparity between donor and recipient. These
       findings may have diagnostic and therapeutic applications in organ
       transplantation.
 DE    Animal  Antigens, CD8/ANALYSIS  Cell Movement/IMMUNOLOGY  CD4-CD8 Ratio
       Graft Rejection/*IMMUNOLOGY  Graft Survival/IMMUNOLOGY  Heart
       Transplantation/*IMMUNOLOGY  Histocompatibility Antigens/*IMMUNOLOGY
       Male  Mice  Mice, Inbred A  Mice, Inbred AKR  Mice, Inbred C3H  Mice,
       Inbred C57BL  Postoperative Period  Support, Non-U.S. Gov't
       T-Lymphocyte Subsets/*IMMUNOLOGY  T-Lymphocytes/IMMUNOLOGY  T4
       Lymphocytes/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).