Document 0108
 DOCN  M9490108
 TI    Determination of L-2-oxothiazolidine-4-carboxylic acid (Procysteine) in
       human plasma by high-performance liquid chromatography.
 DT    9411
 AU    Webb LE; Gechman M; Story K; Baxter Healthcare Corporation, Round Lake,
       IL 60073.
 SO    J Chromatogr B Biomed Appl. 1994 Apr 1;654(2):257-62. Unique Identifier
       : AIDSLINE MED/94319483
 AB    A new high-performance liquid chromatographic (HPLC) method has been
       developed for the determination of the cysteine prodrug,
       L-2-oxothiazolidine-4-carboxylic acid (Procysteine), in human plasma.
       Samples were mixed with 5% metaphosphoric acid and the supernatants
       chromatographed using a reversed-phase analytical column. Procysteine
       was detected spectrophotometrically at 230 nm and quantitated by
       comparison with a standard curve of known amounts of Procysteine
       (20-1000 microM) in plasma. The coefficients of variation for 48.6
       microM and 676 microM control pools were 6.5 and 4.0% respectively (n =
       46). Deviations from the expected concentrations were less than 2%. The
       method has been utilized to evaluate the pharmacokinetics of a wide
       range of oral and intravenous doses.
 DE    Administration, Oral  Chromatography, High Pressure Liquid
       Chromatography, Ion Exchange  Human  HIV Infections/BLOOD  Indicators
       and Reagents  Injections, Intravenous  Quality Control  Reference
       Standards  Respiratory Distress Syndrome, Adult/BLOOD
       Spectrophotometry, Ultraviolet  Thiazoles/ADMINISTRATION &
       DOSAGE/*BLOOD/PHARMACOKINETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).