Document 0554
 DOCN  M9490554
 TI    Immunohistochemical detection of Epstein-Barr virus-encoded latent
       membrane protein in Reed-Sternberg cells and variants of Hodgkin's
       disease.
 DT    9411
 AU    Pinkus GS; Lones M; Shintaku IP; Said JW; Department of Pathology,
       Brigham and Women's Hospital, Boston,; Massachussetts.
 SO    Mod Pathol. 1994 May;7(4):454-61. Unique Identifier : AIDSLINE
       MED/94344943
 AB    A total of 186 specimens of Hodgkin's disease of various histologic
       types (127 nodular sclerosis, 39 mixed cellularity, 14 lymphocyte
       predominance, 3 lymphocyte depleted, and 3 unclassified) were evaluated
       for the presence of latent membrane protein (LMP) and Epstein-Barr virus
       nuclear antigen-2, two Epstein-Barr virus encoded gene products that
       appear to play important roles in cell transformation and oncogenesis.
       Immunoreactivity for LMP was observed in Reed-Sternberg cells and
       variants of 27/39 (69%) cases of mixed cellularity type, 18/127 (14%) of
       nodular sclerosis type, 2/3 cases of lymphocyte depleted type, and 1/3
       cases of unclassified type. All cases of lymphocyte predominance
       Hodgkin's disease were nonreactive for LMP. In cases that were reactive
       for LMP, staining was restricted to Reed-Sternberg cells and variants.
       Other cells within the proliferation, e.g., lymphocytes, histiocytes,
       eosinophils, fibroblasts, etc., were nonreactive. The pattern of
       immunoreactivity for LMP was characterized by strong diffuse cytoplasmic
       staining, occasionally with membrane accentuation and/or paranuclear
       staining. Reactivity for LMP was demonstrated in cryostat sections and
       was also well preserved in paraffin sections of B5- or formalin-fixed
       tissues. Five of six specimens of Hodgkin's disease (4 mixed cellularity
       and 2 nodular sclerosis type) that occurred in HIV-positive patients
       exhibited immunoreactivity for LMP in Reed-Sternberg cells and variants.
       Cryostat section studies for Epstein-Barr virus nuclear antigen-2 using
       monoclonal antibody PE-2 failed to reveal staining for 43 cases (26
       nodular sclerosis, 12 mixed cellularity, and 5 lymphocyte predominance)
       after a 2-h incubation with primary antibody.(ABSTRACT TRUNCATED AT 250
       WORDS)
 DE    Antibodies, Monoclonal  Antigenic Determinants/ANALYSIS  Antigens,
       Viral/*ANALYSIS  Data Interpretation, Statistical  DNA-Binding
       Proteins/ANALYSIS  Frozen Sections  Herpesvirus 4,
       Human/IMMUNOLOGY/*ISOLATION & PURIF  Hodgkin's Disease/*MICROBIOLOGY
       Human  Immunohistochemistry  Paraffin Embedding  Reed-Sternberg
       Cells/*MICROBIOLOGY  Retrospective Studies  Viral Matrix
       Proteins/*ANALYSIS  JOURNAL ARTICLE  REVIEW  REVIEW, MULTICASE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).