Document 0557
 DOCN  M9490557
 TI    Proliferating cell nuclear antigen (PCNA) expression of
       megakaryocytopoiesis in normal human bone marrow and reactive lesions
       with special emphasis on HIV-myelopathy.
 DT    9411
 AU    Thiele J; Titius BR; Kvasnicka HM; Bertsch HP; Erdmann S; Fischer R;
       Institute of Pathology, University of Cologne, FRG.
 SO    Pathol Res Pract. 1994 Jan;190(1):42-50. Unique Identifier : AIDSLINE
       MED/94344844
 AB    A morphometric analysis was performed on bone marrow trephine biopsies
       using sequential double-immunostaining, to evaluate endoreduplicative
       activity of megakaryocytopoiesis. A total of 104 marrow specimens were
       studied with employment of monoclonal antibodies PC10
       (anti-proliferating cell nuclear antigen-PCNA) and Y2/51-CD61
       (anti-platelet glycoprotein IIIa). In addition to the control group
       patients included non-specific inflammatory changes, HIV-myelopathy with
       normal or decreased platelet counts, idiopathic thrombocytopenic purpura
       (ITP), and finally reactive thrombocytosis (TH). To exclude an undue
       overexpression of PCNA, in a comparative pilot study we also applied
       MIB1 (Ki-67 antigen) on normal bone marrow specimens. In accordance with
       the various modalities of cell-cycle marker expression, no significantly
       different findings were disclosed. PCNA-labelling index was relatively
       low, ranging from 0.8 to 1.7% of the total megakaryocytopoiesis
       (promegakaryoblasts to mature platelet-shedding megakaryocytes). A
       significant relationship between megakaryocyte size and PCNA-expression
       was determinable. This implies that some of the cases with a prevalence
       of small megakaryocytes, like ITP, have the tendency to show a higher
       proportion of positively-stained cells. Moreover, this feature confirms
       a hypothesis postulating a decrease in the time for DNA-synthesis
       (S-phase) and a relative prolongation of the G1/G2-phases of the
       cell-cycle at higher ploidy levels (large-sized megakaryocytes). On the
       other hand, it may be speculated that some of the hyperpolyploid giant
       megakaryocytes may have reached their endstage of endoreduplication and
       enter into G0-phase. In comparison with the control group and the other
       entities under study, a significant reduction of PCNA-reactivity was
       recognizable in HIV-myelopathy accompanied by thrombocytopenia.(ABSTRACT
       TRUNCATED AT 250 WORDS)
 DE    Adult  Bone Marrow/*PHYSIOPATHOLOGY  Bone Marrow
       Diseases/*PHYSIOPATHOLOGY  Female  *Hematopoiesis  Human
       Immunohistochemistry/METHODS  Male  *Megakaryocytes  Middle Age  Nuclear
       Proteins/*METABOLISM  Paraparesis, Tropical Spastic/*PHYSIOPATHOLOGY
       Purpura, Thrombocytopenic/PHYSIOPATHOLOGY  Reference Values  Stains and
       Staining  Support, Non-U.S. Gov't  Thrombocytosis/PHYSIOPATHOLOGY
       JOURNAL ARTICLE

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