Document 0575
 DOCN  M9490575
 TI    [Expression of the gene for the gp46 surface glycoprotein from the human
       T-cell leukemia virus type I (HTLV-I) in bacteria]
 DT    9411
 AU    Sankov MN; Bobkov AF; Garaev MM
 SO    Mol Gen Mikrobiol Virusol. 1994 May-Jun;(3):23-5. Unique Identifier :
       AIDSLINE MED/94344161
 AB    A set of recombinant plasmids containing different fragments of HTLV-I
       env gene has been constructed on the basis of pUR290-pUR292 vectors. The
       hybrid proteins containing different fragments of ENV predecessor in the
       C-terminal of beta-galactosidase differed in stability in Escherichia
       coli cells. The presence of N-terminal of ENV predecessor in recombinant
       proteins considerably decreases their resistance to proteases of the
       bacterial cell. Elimination of this fragment led to obtaining of the
       recombinant plasmid pESG coding for the high level of synthesis of the
       env-specific hybrid polypeptide (up to 30% of the total cellular
       protein). This 134 Kda protein is able to interact efficiently with the
       HTLV-I positive sera and may be used in the diagnostic test-systems for
       identification of the HTLV-I infected patients.
 DE    beta-Galactosidase/GENETICS  Cloning, Molecular  English Abstract
       Escherichia coli/GENETICS  Gene Expression  Gene Products, env/*GENETICS
       HTLV-I Antigens/*GENETICS  Plasmids  Retroviridae Proteins,
       Oncogenic/*GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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