Document 0589
 DOCN  M9490589
 TI    [Detection of HTLV-1 provirus DNA in T-cell neoplasms]
 DT    9411
 AU    Takenaka T; Kuribayashi K; Tsukiyama M; Nakamine H; Maeda J; Hiroi K;
       Kuno T; Nakamura Y; Department of Laboratory Medicine, Wakayama Medical
       School.
 SO    Rinsho Byori. 1994 Jul;42(7):725-30. Unique Identifier : AIDSLINE
       MED/94343730
 AB    Using polymerase chain reaction (PCR) amplification and Southern
       hybridization, gag, pol, env and pX region genes of HTLV-1 provirus were
       detected in T-cell malignancy such as adult T-cell leukemia/lymphoma
       (ATLL), mycosis fungoides in leukemic phase (MF) and, CD8-leukemia. The
       gag, pol, and/or env regions, were not amplified in some cases of ATLL,
       which was considered to be induced by mutation, but not deletion.
       However, the pX-1 and pX-2 regions could be amplified in all cases
       examined. As it is suggested that the pX gene plays an important role in
       leukemogenesis, the mutation may not occur in this region gene.
       Interestingly, the pX-2 was amplified in the cases with MF and
       CD8-leukemia as well. The amplified products were hybridized to the
       HTLV-1 pX sequence, even though the products contained DNA fragments
       with a size larger than expected as well as those of an expected size.
       These results indicated the possibility that the virus having sequence
       identical with HTLV-1 pX is integrated in the tumor cells of MF and CD8
       leukemia.
 DE    Base Sequence  DNA, Viral/*ANALYSIS  English Abstract  Human
       HTLV-I/GENETICS/*ISOLATION & PURIF  Leukemia-Lymphoma, T-Cell, Acute,
       HTLV-I-Associated/*MICROBIOLOGY  Molecular Sequence Data  Mycosis
       Fungoides/MICROBIOLOGY  Polymerase Chain Reaction
       Proviruses/GENETICS/*ISOLATION & PURIF  Skin Neoplasms/MICROBIOLOGY
       JOURNAL ARTICLE

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