Document 0629
 DOCN  M9490629
 TI    Evidence that levels of the dimeric cellular transcription factor CP2
       play little role in the activation of the HIV-1 long terminal repeat in
       vivo or following superinfection with herpes simplex virus type 1.
 DT    9411
 AU    Zhong F; Swendeman SL; Popik W; Pitha PM; Sheffery M; Molecular Biology
       Program, Cornell University, Memorial; Sloan-Kettering Cancer Center,
       New York, New York 10021.
 SO    J Biol Chem. 1994 Aug 19;269(33):21269-76. Unique Identifier : AIDSLINE
       MED/94342299
 AB    The dimeric transcription factor CP2 binds a sequence element found near
       the transcription start site of the human immunodeficiency virus (HIV-1)
       long terminal repeat. Several groups have suggested that cellular
       factors binding this element might play a role in modulating HIV-1
       promoter activity in vivo. For example, induction of latent HIV-1 gene
       expression in response to superinfection by herpes simplex virus type 1
       (HSV-1) or cytomegalovirus is thought to be mediated, in part, by
       factors binding the CP2 site. In this report we began to examine
       directly the relationship between CP2 and expression of the HIV-1
       promoter. First, we tested what effect HSV-1 infection of T cells had on
       the cellular levels of CP2. The results showed that HSV-1 infection led
       to a significant reduction in the level of CP2 DNA binding activity and
       protein within 20 h. Next, we tested the effect of overexpressing either
       the wild-type factor or a dominant negative variant of CP2 on HIV-1
       promoter activity in vivo. The results showed that CP2 had little effect
       or slightly repressed HIV-1 promoter activity in vivo. In addition,
       these expression constructs had little effect on the induction of HIV-1
       promoter activity elicited by HSV-1 infection.
 DE    Amino Acid Sequence  Base Sequence  Binding Sites  Cells, Cultured
       DNA-Binding Proteins/*METABOLISM  DNA, Viral/METABOLISM  Herpesvirus 1,
       Human/*METABOLISM  *HIV Long Terminal Repeat  HIV-1/*GENETICS  Molecular
       Sequence Data  NF-kappa B/METABOLISM  *Promoter Regions (Genetics)
       Sequence Homology, Amino Acid  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  T-Lymphocytes/MICROBIOLOGY  Transcription
       Factors/*METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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