Document 0643
 DOCN  M9490643
 TI    Identification of astrovirus serotypes from children treated at the
       Hospitals for Sick Children, London 1981-93.
 DT    9411
 AU    Noel J; Cubitt D; Department of Virology, Hospital for Sick Children,
       London.
 SO    Epidemiol Infect. 1994 Aug;113(1):153-9. Unique Identifier : AIDSLINE
       MED/94341312
 AB    An enzyme immunoassay (EIA) for astrovirus type 1 together with immune
       electronmicroscopy (IEM) was used to type a collection of 162
       astroviruses obtained from 1981-93 from children with diarrhoea. The EIA
       was found to be specific for astrovirus type 1. Astrovirus types 2-4
       were typed by IEM. Astrovirus type 1 was the prevalent serotype 107/125
       (86%), followed by type 3 (8%), type 4 (6%) and type 2 (1%). Six samples
       containing astrovirus could not be typed or detected by EIA because they
       were coated with coproantibodies; 11 others were not identified. Virus
       particles could no longer be detected in 15/162 (9%) samples following
       storage for > or = 2 years. Selected samples containing astrovirus types
       1-4 were passaged in CaCO2 cells and their identity confirmed by one or
       both assays. One sample was shown to have remained viable for 10 years
       when stored as an aqueous suspension at -20 degrees C. Two patients with
       severe combined immune deficiency disease (SCID) were shown to be
       excreting astrovirus type 1 for 32 and 102 days respectively. One child
       was simultaneously shedding rotavirus and the other child was excreting
       adenovirus.
 DE    Astroviruses/*CLASSIFICATION/ULTRASTRUCTURE  Child
       Diarrhea/COMPLICATIONS/*MICROBIOLOGY  Human  Immunoenzyme Techniques
       London  Microscopy, Electron  Microscopy, Immunoelectron  Serotyping
       Severe Combined Immunodeficiency/COMPLICATIONS  Virus
       Diseases/COMPLICATIONS/*MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).