Document 0653
 DOCN  M9490653
 TI    Superantigens activate HIV-1 gene expression in monocytic cells.
 DT    9411
 AU    Fuleihan R; Trede N; Chatila T; Geha RS; Division of Immunology,
       Children's Hospital, Boston,; Massachusetts.
 SO    Clin Immunol Immunopathol. 1994 Sep;72(3):357-61. Unique Identifier :
       AIDSLINE MED/94340810
 AB    Binding of superantigens to MHC class II molecules results in
       transduction of biochemical signals leading to cellular activation and
       gene expression. We demonstrate that the staphylococcal superantigens
       toxic shock syndrome toxin-1 (TSST-1) and staphylococcal enterotoxin A
       (SEA) activate HIV-1-LTR-driven transcription of chloramphenicol acetyl
       transferase in the human monocytic cell line THP-1. Induction of
       HIV-1-LTR-driven transcription in THP-1 cells by superantigens was
       associated with the induction of nuclear factor-kappa B DNA-binding
       activity. Superantigens also increased viral protein secretion from the
       granulocyte-macrophage colony-stimulating factor-pretreated chronically
       infected human monocytic cell line U1. Induction of HIV-1 gene
       expression in monocytic cells by superantigens occurred via tumor
       necrosis factor-alpha-dependent and -independent mechanisms. Our results
       suggest that superantigens and other MHC class II ligands may activate
       HIV-1 gene expression in monocytes/macrophages.
 DE    Base Sequence  Cell Line  Electrophoresis/METHODS  Histocompatibility
       Antigens Class II/IMMUNOLOGY  Human  HIV Core Protein p24/BIOSYNTHESIS
       HIV Infections/*IMMUNOLOGY/*MICROBIOLOGY  HIV Long Terminal Repeat
       HIV-1/*GENETICS/IMMUNOLOGY  Molecular Sequence Data
       Monocytes/MICROBIOLOGY  NF-kappa B/PHYSIOLOGY  Superantigens/*IMMUNOLOGY
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       Transfection/GENETICS  Tumor Necrosis Factor/PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).