Document 0655
 DOCN  M9490655
 TI    Virus production and spontaneous cell proliferation in HTLV-I-infected
       lymphocytes.
 DT    9411
 AU    Mann DL; Martin P; Hamlin-Green G; Nalewaik R; Blattner W; Laboratory of
       Viral Carcinogenesis, Program Resources,; Inc./DynCorp., National Cancer
       Institute Frederick Cancer; Research and Development Center, Maryland
       21702-1201.
 SO    Clin Immunol Immunopathol. 1994 Sep;72(3):312-20. Unique Identifier :
       AIDSLINE MED/94340804
 AB    Cultured peripheral blood lymphocytes (PBL) from HTLV-I-infected
       individuals proliferate in the absence of added mitogens and/or
       cytokines. In an attempt to answer questions regarding the activating
       signals for cells and virus, antibodies that react with cell surface
       components that are known to regulate cell activation and antibodies
       reacting with viral proteins were added to cultures of PBL from
       HTLV-I-infected, disease-free individuals. Spontaneous proliferation and
       virus production increased in the presence of antibodies reacting with
       CD3 and alpha/beta T cell receptors (TCR) while antibodies to HLA class
       II and viral proteins had no effect. Addition of HLA class I antibodies
       shut down virus production and cell proliferation. These observations
       indicate that both virus and cell activation may occur through the
       alpha/beta TCR on the infected cell. Cyclosporin A, however, markedly
       decreased cell proliferation but had only a modest suppressive effect on
       virus production. Thus, the uncoupling of cell proliferation from virus
       production by cyclosporin A suggests the possibility that the signal
       transduction pathways for these two events are different.
 DE    Antibodies, Monoclonal  Antigens, Surface/IMMUNOLOGY  Base Sequence
       Cells, Cultured  Cyclosporine/PHARMACOLOGY  DNA, Viral/GENETICS  Human
       HTLV-I/DRUG EFFECTS/IMMUNOLOGY/*PHYSIOLOGY  HTLV-I Antigens/IMMUNOLOGY
       HTLV-I Infections/*IMMUNOLOGY/*MICROBIOLOGY  Lymphocyte
       Transformation/DRUG EFFECTS/*IMMUNOLOGY  Molecular Sequence Data
       Polymerase Chain Reaction  RNA, Viral/BIOSYNTHESIS/GENETICS  Support,
       U.S. Gov't, P.H.S.  T-Lymphocyte
       Subsets/CYTOLOGY/*IMMUNOLOGY/*MICROBIOLOGY  Virus Replication/DRUG
       EFFECTS/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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