Document 0666
 DOCN  M9490666
 TI    Simultaneous determination of primaquine and carboxyprimaquine in plasma
       using high-performance liquid chromatography with electrochemical
       detection.
 DT    9411
 AU    Dean RA; Ochieng W; Black J; Queener SF; Bartlett MS; Dumaual NG;
       Department of Pathology and Laboratory Medicine, School of; Medicine,
       Indiana University, Indianapolis.
 SO    J Chromatogr B Biomed Appl. 1994 Apr 22;655(1):89-96. Unique Identifier
       : AIDSLINE MED/94340105
 AB    A selective and sensitive high-performance liquid chromatographic method
       with electrochemical detection is described for the simultaneous
       quantitation of primaquine and carboxyprimaquine, its primary
       metabolite, in plasma. After addition of internal standard, plasma was
       deproteinized by addition of acetonitrile. Nitrogen-dried supernatants,
       resuspended in mobile phase were analyzed on a C8 reversed-phase column.
       Limits of detection for primaquine and carboxyprimaquine were 2 and 5
       ng/ml with quantitation limits of 5 and 20 ng/ml, respectively. None of
       47 tested antimicrobial agents interfered. In contrast to previously
       reported methods, the assay sensitivity and specificity are sufficient
       to permit quantitation of primaquine in plasma for pharmacokinetics
       following low dose (30 mg, base) oral administration of primaquine,
       typically used in the treatment of malaria and Pneumocystis carinii
       pneumonia.
 DE    Acquired Immunodeficiency Syndrome/DRUG THERAPY  Chromatography, High
       Pressure Liquid  Drug Combinations  Electrochemistry  Human  Indicators
       and Reagents  Primaquine/*ANALOGS & DERIVATIVES/BLOOD/PHARMACOKINETICS
       Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).