Document 0669
 DOCN  M9490669
 TI    Follow-up investigation of indeterminate western blot results for
       antibody to human immunodeficiency virus type 1.
 DT    9411
 AU    Lee JH; Department of Clinical Pathology, Tri-Service General Hospital,;
       Taipei, Taiwan R.O.C.
 SO    J Formos Med Assoc. 1994 Apr;93(4):283-8. Unique Identifier : AIDSLINE
       MED/94339747
 AB    A total of 1,066 serum samples from 911 individuals with repeatedly
       reactive enzyme-linked immunosorbent assay (ELISA) for antibody to the
       human immunodeficiency virus type 1 (HIV-1) were enrolled for
       confirmatory HIV-1 infection diagnosis during the three years from 1990
       to 1993. According to the interpretation criteria for the anti-HIV
       Western blot test recommended by the Centers for Disease Control, 38
       (4.2%) were Western blot-positive, 110 (12.1%) were Western
       blot-negative, and 763 (83.7%) were Western blot-indeterminate. The most
       common band patterns of indeterminate Western blot results were
       antibodies to gag gene product only (667/763, 87.5%) which included p18
       only (180, 23.6%), p18 plus others (521, 68.3%), p25 only (55, 7.2%),
       and p25 plus others (212, 27.8%). Eighty-three individuals with
       indeterminate Western blot results were followed-up and new serum
       samples were collected. None of the follow-up samples became positive.
       When band patterns changed, they usually did so within the specific
       category (either gag, pol, or env), such as a change from p18 to its
       precursor p55. All of the indeterminate specimens tested by particle
       agglutination assay showed negative reaction. In conclusion, an
       indeterminate result should not been seen as final; laboratory testing
       is required on the follow-up specimens.
 DE    Agglutination Tests  AIDS Serodiagnosis  *Blotting, Western
       Enzyme-Linked Immunosorbent Assay  Human  HIV Antibodies/*ANALYSIS
       HIV-1/*IMMUNOLOGY  JOURNAL ARTICLE

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