Document 0673
 DOCN  M9490673
 TI    Inhibition of HIV-1 multiplication in a human CD4+ lymphocytic cell line
       expressing antisense and sense RNA molecules containing HIV-1 packaging
       signal and Rev response element(s).
 DT    9411
 AU    Cohli H; Fan B; Joshi RL; Ramezani A; Li X; Joshi S; Department of
       Microbiology, University of Toronto, Ontario,; Canada.
 SO    Antisense Res Dev. 1994 Spring;4(1):19-26. Unique Identifier : AIDSLINE
       MED/94339688
 AB    Moloney murine leukemia virua (MoMuLV)-derived retroviral vectors were
       engineered to express human immunodeficiency virus type 1 (HIV-1)
       packaging (psi) signal and Rev response element (RRE) sequences in
       either sense or antisense orientation. The RRE sequences were expressed
       under the control of the herpes simplex virus (HSV) thymidine kinase
       (tk) promoter fused to the HIV-1 trans-activation-responsive (TAR)
       element, while the psi signal sequences were expressed under control of
       the HSV tk promoter. Both RRE and psi signal sequences were expressed as
       part of the 3' untranslated region of the neomycin phosphotransferase
       (neo) mRNA. The constructs were used to transfect/infect packaging cell
       lines and the retroviral vector particles released were used to infect a
       human CD4+ lymphocyte-derived MT4 cell line. The stable MT4
       transformants, harboring proviral vector DNA expressing one to two
       copies of HIV-1 RRE and psi signal in either antisense or sense
       orientation, were each tested for their susceptibility to HIV-1
       infection. Compared to the results obtained with the control cells
       lacking any of the test DNA sequences, the rate of HIV-1 production
       remained unaltered in RRE1+ (sense RNA containing a single copy of RRE)
       RNA-containing cells, whereas it was delayed in cells expressing both
       RRE2+ (sense RNA containing two copies of RRE) and RRE1- (antisense RNA
       containing a single copy of RRE) RNA-expressing cells. In cells
       expressing HIV-1 psi signal, HIV-1 production remained unaltered in psi
       + RNA-expressing cells, whereas it was delayed by up to 30 days in psi -
       RNA-expressing cells.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Base Sequence  Cell Line  Genes, env/*GENETICS  Genetic Vectors  Human
       HIV-1/GENETICS/*PHYSIOLOGY  Molecular Sequence Data  Plasmids
       Polymerase Chain Reaction  Promoter Regions (Genetics)  Regulatory
       Sequences, Nucleic Acid/*GENETICS  RNA, Antisense/*GENETICS  RNA,
       Viral/*GENETICS  Support, Non-U.S. Gov't  Transfection  T4
       Lymphocytes/*MICROBIOLOGY  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).