Document 0694
 DOCN  M9490694
 TI    Magic Lite Chlamydia immunoassay in urogenital samples and urine versus
       chlamydial culture.
 DT    9411
 AU    Bygdeman SM; Johansson M; Jonasson J; Lidbrink P; Nilsson U; Teichert C;
       Viktorsson S; Karolinska Institute, Department of Clinical
       Bacteriology,; Huddinge Hospital, Stockholm, Sweden.
 SO    Int J STD AIDS. 1994 May-Jun;5(3):207-11. Unique Identifier : AIDSLINE
       MED/94339225
 AB    Magic Lite Chlamydia assay (commercial test kit for the identification
       of Chlamydia trachomatis) was evaluated on urogenital samples and urine
       with chlamydial culture as the reference method. The sediment of the
       transportation buffer of specimens which were Magic Lite positive but
       culture negative or toxic was investigated for elementary bodies with
       fluorescein-labelled anti-chlamydial antibodies. The prevalence of
       chlamydial infection among the 577 men investigated was 13.7% as
       estimated by culture and direct immunofluorescence and 6.4% among the
       173 women. In order to improve the sensitivity a cut-off value below
       that recommended by the manufacturer was used. The sensitivity of Magic
       Lite in male urethral specimens was then 60.8% and that in female
       urethral/cervical specimens 90.9%. The specificity was 99.6% and 100%,
       respectively. In urine specimens the sensitivity of Magic Lite was 63.3%
       (men) and 63.6% (women). The specificity was 99.4% and 100%,
       respectively. The sensitivity of Magic Lite on male urethral specimens
       was related to the number of inclusion bodies per well in culture and it
       was higher among men attending with clinical findings of urethritis
       (69%) than among asymptomatic men sampled as a screening procedure (36%)
       (P < 0.05). Corresponding differences between the sexes and between
       those with and without symptoms were not noted for Magic Lite applied on
       urine samples.
 DE    Adolescence  Adult  Aged  Chlamydia trachomatis/IMMUNOLOGY/*ISOLATION &
       PURIF  Chlamydia Infections/EPIDEMIOLOGY/*MICROBIOLOGY  Evaluation
       Studies  Female  Fluorescent Antibody Technique  Human  Male  Middle Age
       Predictive Value of Tests  Prevalence  Reagent Kits,
       Diagnostic/*STANDARDS  Sensitivity and Specificity  Urine/*MICROBIOLOGY
       Urogenital System/*MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).