Document 0009
 DOCN  M94A0009
 TI    Characterization of lesional psoriatic skin T lymphocyte clones.
 DT    9412
 AU    Barna M; Snijdewint FG; van der Heijden FL; Bos JD; Kapsenberg ML;
       Department of Dermatology, Academisch Medisch Centrum, University; of
       Amsterdam, The Netherlands.
 SO    Acta Derm Venereol Suppl (Stockh). 1994;186:9-11. Unique Identifier :
       AIDSLINE MED/94353903
 AB    T cells are considered to play a role in the pathomechanism of
       psoriasis. Therefore we investigated the cytokine production patterns of
       T cell clones that were randomly prepared from chronic plaque psoriasis
       lesions of 2 patients. 67% of the 49 T lymphocyte clones (TLC) expressed
       CD4 and 33% expressed CD8 (ratio 2:1), while gamma delta-TCR expression
       was absent. The production of IL-4, IFN-gamma, IL-2 and IL-6 was
       measured in supernatants of TLC following PHA plus PMA stimulation.
       Different groups of clones could be distinguished according to their
       IL-4/IFN-gamma production ratio. In addition to Th0 cells (low IL-4/low
       IFN-gamma), low IL-4/high IFN-gamma producers as well as high IL-4/low
       IFN-gamma producing clones were found, suggesting the presence of Th1-
       and Th2-like subsets. Upon stimulation, all TLC secreted low levels of
       IL-2 whereas a minority of the TLC secreted low levels of IL-6. These
       results may imply that T cells in psoriasis lesions do not show shifts
       towards either a Th1 or a Th2 cytokine production profile.
 DE    Chronic Disease  Clone Cells  Cytokines/*METABOLISM  CD4-CD8 Ratio
       Human  Immunophenotyping  Interferon Type II/METABOLISM
       Interleukin-4/METABOLISM  Male  Psoriasis/*IMMUNOLOGY/PATHOLOGY  Random
       Allocation  Receptors, Antigen, T-Cell, gamma-delta/ANALYSIS
       Skin/*PATHOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes/*IMMUNOLOGY/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).