Document 0015
 DOCN  M94A0015
 TI    Occurrence of multiple JC virus variants with distinctive regulatory
       sequences in the brain of a single patient with progressive multifocal
       leukoencephalopathy.
 DT    9412
 AU    Yogo Y; Guo J; Iida T; Satoh K; Taguchi F; Takahashi H; Hall WW;
       Nagashima K; Department of Viral Infection, University of Tokyo, Japan.
 SO    Virus Genes. 1994 Mar;8(2):99-105. Unique Identifier : AIDSLINE
       GENBANK/D11366
 AB    We established 99 JC virus (JCV) DNA clones directly from the brain of a
       single patient with progressive multifocal leukoencephalopathy (PML).
       Based upon restriction patterns, the cloned viral DNAs were classified
       into two major groups (NY-1A and -1B) containing 53 and 35 clones,
       respectively, and several minor groups containing one or a few clones.
       The regulatory sequences of representative clones were compared with the
       archetypal regulatory sequence, which has been detected in JCV DNAs
       cloned from the urine of healthy and nonimmunosuppressed individuals.
       The regulatory sequence of NY-1B had the two structural features common
       to most PML-type regulatory regions, duplication and deletion of
       specific segments in the archetypal sequence, while that of NY-1A
       contained a small deletion and an insertion of a 29-bp sequence
       originating from the early region of the JCV genome. A regulatory region
       similar to that of NY-1A has never been detected in JCV isolates
       obtained thus far.
 DE    Adult  AIDS-Related Opportunistic Infections/COMPLICATIONS  Base
       Sequence  Brain/*MICROBIOLOGY  Capsid/GENETICS  Cloning, Molecular
       Genes, Viral/GENETICS  Human  Leukoencephalopathy, Progressive
       Multifocal/COMPLICATIONS/  *MICROBIOLOGY  Male  Molecular Sequence Data
       Polyomavirus hominis 2/*GENETICS  Regulatory Sequences, Nucleic
       Acid/*GENETICS  Restriction Fragment Length Polymorphisms  Restriction
       Mapping  Variation (Genetics)/*GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).