Document 0028
 DOCN  M94A0028
 TI    [Riboprobes: an alternative in the detection of viral sequences]
 DT    9412
 AU    Gutierrez-Escolano AL; Castro-Rodriguez EM; Nunez-de Caceres RM;
       Soler-Claudin C; Fernandez-Tomas C; Laboratorio del Departamento de
       Patologia Experimental, Centro; de Investigacion y de Estudios
       Avanzados, Instituto Politecnico; Nacional (CINVESTAV-IPN), Mexico.
 SO    Salud Publica Mex. 1994 Mar-Apr;36(2):140-7. Unique Identifier :
       AIDSLINE MED/94353286
 AB    The use of riboprobes for the detection of RNA viral sequences is
       analyzed. Subgenomic fragments of cDNA from poliovirus type 2, dengue
       virus type 4 and human immunodeficiency virus type 1, were inserted
       downstream SP6 and/or T7 promoters in the transcription vectors pGEM-4z
       or pSP64. RNAs obtained by in vitro transcription in the presence of UTP
       infinity (32P) were used as probes for the detection of RNA viral
       sequences from infected cell lines in slot and Northern blot assays. The
       poliovirus riboprobe (P2-221) was able to detect specific viral
       sequences; thus, it could be used for the detection of the virus in
       serum, as well as in residual waters. The human immunodeficiency virus
       riboprobe (HIV-378), detected viral sequences poly A+RNA from infected
       cells; thus it can be used as a confirmatory test or as a tool in basic
       research. Finally, the dengue virus riboprobes (D4-2819 and D4-1134)
       detected specifically dengue 4 virus; however the sensitivity of the
       detection could be significantly improved amplifying viral sequences by
       the polymerase chain reaction (PCR) prior to probe hybridization.
 DE    Blotting, Northern  Cells, Cultured  Dengue/DIAGNOSIS  Dengue
       Virus/*GENETICS  English Abstract  Human  HIV Infections/DIAGNOSIS
       HIV-1/*GENETICS  Nucleic Acid Hybridization  Plasmids/GENETICS
       Poliomyelitis/DIAGNOSIS  Polioviruses, Human 1-3/*GENETICS  *RNA
       Probes/BIOSYNTHESIS/DIAGNOSTIC USE  RNA,
       Viral/ANALYSIS/GENETICS/*ISOLATION & PURIF  Support, Non-U.S. Gov't
       Virus Cultivation  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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