Document 0065
 DOCN  M94A0065
 TI    HIV-1 reverse transcription. A termination step at the center of the
       genome.
 DT    9412
 AU    Charneau P; Mirambeau G; Roux P; Paulous S; Buc H; Clavel F; Unite
       d'Oncologie Virale, CNRS URA 1157, Institut Pasteur,; Paris, France.
 SO    J Mol Biol. 1994 Sep 2;241(5):651-62. Unique Identifier : AIDSLINE
       MED/94351714
 AB    During HIV-1 reverse transcription, the plus-strand of viral DNA is
       synthesized as two discrete segments. We show here that synthesis of the
       upstream segment terminates at the center of the genome after an 88 or
       98 nucleotide strand displacement of the downstream segment, initiated
       at the central polypurine tract. Thus, the final structure of
       unintegrated linear HIV-1 DNA includes a central plus-strand overlap. In
       vitro reconstitution using only purified reverse transcriptase with
       appropriate DNA hybrids gave rise to efficient and accurate termination,
       which was dramatically amplified in the context of strand displacement.
       Mutation of the sequence immediately upstream of the termination sites
       almost completely abolished termination both in infected cells and in
       vitro. This mutation profoundly impaired replication of HIV-1. We
       conclude that proper central plus-strand termination, mediated by a
       novel cis-active termination sequence, is a key step in HIV-1
       replication.
 DE    Base Sequence  Cell Line, Transformed  Conserved Sequence  DNA,
       Viral/BIOSYNTHESIS  Human  HIV-1/*GENETICS  Models, Genetic  Molecular
       Sequence Data  Mutagenesis, Site-Directed  Reverse
       Transcriptase/*METABOLISM  RNA, Viral/*GENETICS  Sequence Analysis, DNA
       Support, Non-U.S. Gov't  Terminator Regions (Genetics)/*GENETICS
       Transcription, Genetic/*PHYSIOLOGY  Virus Replication/PHYSIOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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