Document 0071
 DOCN  M94A0071
 TI    Virologic, immunologic, and clinical evaluation of human
       immunodeficiency virus antibody status of symptom-free children born to
       infected mothers.
 DT    9412
 AU    Wiznia AA; Lambert G; Dobrosyzcki J; Porricolo M; Chelyapov N; Israeli
       V; Brunell P; Conroy J; Liu KN; Baron P; et al; Division of Pediatric
       Infectious Disease, Bronx Lebanon Hospital; Center, New York 10457.
 SO    J Pediatr. 1994 Sep;125(3):352-5. Unique Identifier : AIDSLINE
       MED/94351418
 AB    STUDY OBJECTIVE: To determine the prevalence of infection by the human
       immunodeficiency virus (HIV) in a population of symptom-free children
       who were born to HIV-infected mothers and who subsequently underwent
       seroreversion from an HIV antibody-positive to an HIV antibody-negative
       status. DESIGN: Cohort. SETTING: Pediatric HIV program in a community
       setting. PATIENTS: We used HIV DNA polymerase chain reaction (PCR) and
       coculture to detect the presence or absence of HIV in peripheral blood
       mononuclear cells of 134 children aged 6 to 53 months. All children had
       HIV antibody at birth and underwent a subsequent seroreversion to
       antibody-negative status. RESULTS: In 134 children with HIV
       antibody-negative status, 219 of 220 culture results and 242 of 247
       HIV-1 DNA PCR assay results were negative. Six positive laboratory
       results were obtained for six different children, each of whom had
       negative results on multiple assays. For HIV-infected children, 56 of 62
       cultures and 99 of 104 PCR evaluations showed positive results. There
       was no clinical or laboratory evidence of HIV infection in the group
       with HIV antibody-negative status. CONCLUSION: We were unable to find
       evidence of latent HIV type 1 infection in this cohort of symptom-free
       children who underwent seroreversion to HIV antibody-negative status.
       The loss of maternal HIV antibody in these children indicates the
       absence of HIV infection. False-positive PCR and culture results
       occurred sporadically, indicating that repeated analysis of HIV
       seropositivity in infants and children is necessary.
 DE    Adolescence  Child  Child, Preschool  Cohort Studies  DNA,
       Viral/ANALYSIS/GENETICS  Female  Human  HIV Antibodies/*BLOOD  HIV
       Infections/*CONGENITAL/IMMUNOLOGY/MICROBIOLOGY  HIV
       Seronegativity/*IMMUNOLOGY  HIV-1/GENETICS/*IMMUNOLOGY/ISOLATION & PURIF
       IgA/BLOOD  IgG/BLOOD  IgM/BLOOD  Infant  Infant, Newborn  Leukocyte
       Count  Polymerase Chain Reaction  Pregnancy  Pregnancy Complications,
       Infectious/IMMUNOLOGY/*MICROBIOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes, Cytotoxic/PATHOLOGY  T4 Lymphocytes/PATHOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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