Document 0079
 DOCN  M94A0079
 TI    Use of TrpE fusion protein to identify antigenic domains within the BIV
       envelope protein.
 DT    9412
 AU    Chen P; Liu ZQ; Wood C; Department of Microbiology, University of
       Kansas, Lawrence 66045.
 SO    J Virol Methods. 1994 May;47(3):331-43. Unique Identifier : AIDSLINE
       MED/94351053
 AB    Nine different recombinant clones spanning various regions of the bovine
       immunodeficiency-like virus (BIV) envelope gene open reading frame were
       generated. These clones span the entire external glycoprotein as well as
       the transmembrane glycoprotein region. These proteins were expressed as
       fusions to the TrpE protein in E. coli. The levels of recombinant
       protein expressed varied, some clones expressed enough protein that can
       be detected in a Coomassie blue-stained gel, whereas other proteins
       could only be detected by Western blot analyses. A recombinant env
       protein representing the extracellular domain of the env protein was
       detected by BIV-infected bovine sera. In addition, a 134 amino acid
       peptide which may represent a major immunoreactive epitope was
       identified. This peptide is located at the amino terminus of the
       transmembrane glycoprotein and was specifically recognized by all
       BIV-infected calf sera tested. The identification of this epitope and
       the use of recombinant envelope protein will enable us to develop a more
       effective screening test to study the epidemiology of BIV infection.
 DE    Animal  Antigenic Determinants/*ANALYSIS  Cattle  Cloning, Molecular
       Escherichia coli/GENETICS  Immunoblotting  Immunodeficiency Virus,
       Bovine/GENETICS/*IMMUNOLOGY  Lentivirus Infections/MICROBIOLOGY
       Recombinant Fusion Proteins/GENETICS/IMMUNOLOGY  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  Viral Envelope
       Proteins/GENETICS/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).