Document 0142
 DOCN  M94A0142
 TI    Stromal cell lines derived from LP-BM5 murine leukemia virus-infected
       long-term bone marrow cultures impair hematopoiesis in vitro.
 DT    9412
 AU    Tse KF; Morrow JK; Hughes NK; Gallicchio VS; Department of Internal
       Medicine, University of Kentucky Medical; Center, Lexington.
 SO    Blood. 1994 Sep 1;84(5):1508-18. Unique Identifier : AIDSLINE
       MED/94348074
 AB    Murine acquired immunodeficiency syndrome (MAIDS) induced by defective
       LP-BM5 murine leukemia virus is a disease with many similarities to
       human AIDS. Previous studies indicated that the depressed hematopoiesis
       observed in LP-BM5-infected marrow cultures may be attributable to a
       defect of hematopoietic stroma. We report here the generation of
       permanent stromal cell lines from noninfected and LP-BM5-infected marrow
       cultures. Retrovirus infection was confirmed by polymerase chain
       reaction for viral genome. The ability of these cell lines to support in
       vitro hematopoiesis was studied. Results indicated that, when cocultured
       with normal or infected nonadherent mononuclear cells, noninfected cell
       lines efficiently supported the production of hematopoietic precursors,
       whereas viral-infected cell lines induced suppression of both normal and
       viral-infected progenitors. Expression of cytokine genes in stromal cell
       lines was also examined. All cell lines expressed equivalent levels of
       transcripts for stem cell factor and tumor necrosis factor alpha.
       However, infection was associated with higher levels of interleukin-4
       and transforming growth factor beta 1 transcript expression. These
       findings suggest that infected stromal cell lines exhibit a defective
       hematopoietic microenvironment that produced altered cytokine expression
       resulting in faulty hematopoiesis. Further characterization of the
       defective cell lines should prove valuable for studies of the
       pathogenesis of murine AIDS.
 DE    Animal  Base Sequence  Bone Marrow/*PATHOLOGY  Cell Line  Cell
       Separation  Cells, Cultured  Colony-Forming Units Assay
       Cytokines/*BIOSYNTHESIS  Defective Viruses/GENETICS/ISOLATION &
       PURIF/ULTRASTRUCTURE  DNA Primers  *Gene Expression  Genes, gag
       *Hematopoiesis  Hematopoietic Cell Growth Factors/BIOSYNTHESIS
       Hematopoietic Stem Cells/CYTOLOGY/*PATHOLOGY/PHYSIOLOGY
       Interleukin-3/BIOSYNTHESIS  Interleukin-4/BIOSYNTHESIS  Leukemia
       Viruses, Murine/*GENETICS/ISOLATION & PURIF/  ULTRASTRUCTURE  Mice
       Mice, Inbred C57BL  Molecular Sequence Data  Murine Acquired
       Immunodeficiency Syndrome/MICROBIOLOGY  Nuclear
       Membrane/MICROBIOLOGY/ULTRASTRUCTURE  Polymerase Chain Reaction
       Support, U.S. Gov't, Non-P.H.S.  Transforming Growth Factor
       beta/BIOSYNTHESIS  Tumor Necrosis Factor/BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).