Document 0162
 DOCN  M94A0162
 TI    Normal immune function and inability to isolate virus in culture in an
       individual with long-term human immunodeficiency virus type 1 infection.
 DT    9412
 AU    Greenough TC; Somasundaran M; Brettler DB; Hesselton RM; Alimenti A;
       Kirchhoff F; Panicali D; Sullivan JL; Department of Pediatrics,
       University of Massachusetts Medical; School, Worcester 01605.
 SO    AIDS Res Hum Retroviruses. 1994 Apr;10(4):395-403. Unique Identifier :
       AIDSLINE MED/94347464
 AB    A detailed, longitudinal study was undertaken to investigate the
       immunological and virological features of an individual with hemophilia
       infected with human immunodeficiency virus type-1 (HIV-1) for 10 years
       without disease. Methods applied to serial samples of peripheral blood
       included Western blot analysis, neutralizing antibody assays,
       antibody-dependent cell-mediated cytotoxicity (ADCC) titration, HIV-1
       specific cytotoxic T lymphocyte (CTL) assays, viral cultures, and PCR
       with sequence analysis of viral regulatory genes. Strong antibody
       responses against HIV-1 antigens as measured by Western blot and ADCC
       assays have persisted throughout infection. Repeated attempts to isolate
       HIV-1 using sensitive culture techniques and to demonstrate viremia with
       standard PCR methods have failed. Using the booster PCR technique, a
       period of viremia in peripheral blood mononuclear cells was
       demonstrated. Concurrent with detection of circulating virus, titers of
       neutralizing antibodies and circulating HIV-1-specific CTLs became
       measurable. Sequencing studies of a portion of the viral genome showed
       no significant abnormalities of the regulatory genes. In this
       individual, the combination of low viral load in the peripheral blood
       and a strong, responsive immune system is associated with long-term,
       disease-free coexistence with HIV-1 infection.
 DE    Antibody-Dependent Cell Cytotoxicity  Case Report  DNA,
       Viral/GENETICS/ISOLATION & PURIF  Hemophilia/COMPLICATIONS  Human  HIV
       Antibodies/BLOOD  HIV Infections/COMPLICATIONS/*IMMUNOLOGY/MICROBIOLOGY
       *HIV-1/GENETICS/ISOLATION & PURIF  Male  Middle Age  Phenotype
       Polymerase Chain Reaction  Support, U.S. Gov't, P.H.S.  T-Lymphocytes,
       Cytotoxic/IMMUNOLOGY  Time Factors  Viremia/MICROBIOLOGY  Virus
       Cultivation  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).