Document 0164
 DOCN  M94A0164
 TI    Cell line-dependent release of HIV-like gag particles after infection of
       mammalian cells with recombinant vaccinia viruses.
 DT    9412
 AU    Dru A; Ludosky MA; Cartaud J; Beaud G; Institut Jacques Monod, Paris,
       France.
 SO    AIDS Res Hum Retroviruses. 1994 Apr;10(4):383-90. Unique Identifier :
       AIDSLINE MED/94347462
 AB    We investigated the production of Gag particles by Vero, CV-1, or 1D
       cells infected with different vaccinia virus recombinants expressing HIV
       gag or gag-pol genes. Immunoblots of (centrifuged) culture media from 1D
       cells infected with vMM5, a vaccinia virus recombinant expressing the
       HIV-2 gag-pol genes, revealed the presence of abundant particles that
       contained (mostly processed) Gag antigens. In contrast, Gag particles
       were found only in low amounts in the culture medium from Vero cells
       infected with the same HIV gag-pol vaccinia virus recombinant; the Gag
       precursor remained associated with the infected Vero cells and was
       efficiently processed. This low excretion of Gag particles after
       infection of Vero cells with vMM5 was also demonstrated by assays of
       reverse transcriptase activity in the pellet of centrifuged culture
       medium. Cell fractionation showed that Gag proteins were predominantly
       found in the membrane fraction from both 1D and Vero cells. Electron
       microscopy observations of 1D or of Vero cells infected with vMM5
       vaccinia virus recombinant revealed in both cases the presence of
       particles budding at the plasma membrane. However, the shape of the
       budding particles was different in the two cell lines, with immature
       forms present in the membrane from the infected Vero cells. An
       inefficient excretion of Gag particles was also observed after infection
       of Vero cells with different vaccinia virus recombinants expressing
       either an uncleaved HIV-2 Gag protein or the HIV-1 gag-pol genes, as
       judged both by immunoblot and reverse transcriptase activity
       assays.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Animal  Cell Line  Gene Expression  Gene Products,
       gag/*BIOSYNTHESIS/GENETICS  Genes, gag  Genes, pol  HIV-1/*GENETICS
       HIV-2/*GENETICS  Mice  Microscopy, Electron  Myristic Acids/METABOLISM
       Protein Precursors/BIOSYNTHESIS/GENETICS  Protein Processing,
       Post-Translational  Recombination, Genetic  Reverse
       Transcriptase/METABOLISM  Support, Non-U.S. Gov't  Vaccinia
       Virus/*GENETICS  Vero Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).