Document 0168
 DOCN  M94A0168
 TI    Construction and in vitro properties of HIV-1 mutants with deletions in
       nonessential genes.
 DT    9412
 AU    Gibbs JS; Regier DA; Desrosiers RC; Harvard Medical School, New England
       Regional Primate Research; Center, Southborough, Massachusetts
       01772-9102.
 SO    AIDS Res Hum Retroviruses. 1994 Apr;10(4):343-50. Unique Identifier :
       AIDSLINE MED/94347458
 AB    Auxiliary genes that are not essential for viral replication in cell
       culture are found in all known lentiviruses. The nonessential auxiliary
       genes of HIV-1 are vif, vpr, vpu, and nef. Sequences within the upstream
       region of U3 in the LTR are also not required for virus replication in
       cell culture. We constructed a panel of 23 mutants with single and
       combination deletions in these regions in the wild-type HIV-1 infectious
       molecular clone NL4-3. Deletion of the vpu, vpr, and nef genes and the
       U3 upstream sequence (US), individually or in combinations, did not
       appreciably alter virus replication in either chimpanzee PBMCs, human
       PBMCs, or in the B/T cell hybrid line CEMx174. In contrast, deletion of
       the vif gene dramatically delayed virus replication in all three cell
       types. This collection of HIV-1 deletion mutants will be useful for
       elucidating the functions of these genes and for investigating antiviral
       immunity in animal models.
 DE    Animal  Base Sequence  Cell Line  Chimpansee troglodytes  DNA,
       Viral/GENETICS  *Gene Deletion  Genes, nef  Genes, vif  Genes, vpr
       Genes, vpu  *Genes, Viral  Human  HIV Long Terminal Repeat
       HIV-1/*GENETICS/PHYSIOLOGY  In Vitro  Leukocytes,
       Mononuclear/MICROBIOLOGY  Molecular Sequence Data  Mutagenesis  Support,
       U.S. Gov't, P.H.S.  Virus Replication/GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).