Document 0178
 DOCN  M94A0178
 TI    Alkaline phosphatase band-10 fraction as a possible surrogate marker for
       human immunodeficiency virus type 1 infection in children [see comments]
 DT    9412
 AU    Murthy VV; Calvelli TA; Rubinstein A; Karmen A; Department of Laboratory
       Medicine, Albert Einstein College of; Medicine, Bronx, NY 10461.
 SO    Arch Pathol Lab Med. 1994 Sep;118(9):873-7. Unique Identifier : AIDSLINE
       MED/94361595
 CM    Comment in: Arch Pathol Lab Med 1994 Sep;118(9):868-9
 AB    We report the utility of a possible lymphocyte fraction of alkaline
       phosphatase (ALP band-10) activity in serum to predict human
       immunodeficiency virus type 1 (HIV-1) infection in children born to
       HIV-1-seropositive mothers. The presence of ALP band 10 in serum
       consistently correlated with HIV-1 infection status as judged by
       positive HIV-1 culture, two consecutive HIV-1 p24 antigen results
       greater than 30 pg/mL in serum, and the subsequent confirmation of
       seroconversion to HIV-1 antibody after clearance of maternal IgG
       anti-HIV-1 antibody ascertained between 15 to 24 months post partum.
       Infection with HIV-1 was correctly identified in 31 samples from 18
       patients ranging in age between 0.1 to 10 years; the absence of similar
       infection was noted in 14 samples from nine patients who served as
       controls and whose serum samples did not exhibit ALP band-10 activity.
       This ability of serum ALP band-10 activity to predict HIV-1 infection
       status in children as young as 2 months may be useful as a surrogate
       marker for early identification of HIV-1 infection in infants born to
       HIV-1-seropositive women long before the clearance of maternal
       anti-HIV-1 antibodies can be ascertained.
 DE    Alkaline Phosphatase/*BLOOD  Biological Markers/BLOOD  Child  Child,
       Preschool  Human  HIV Infections/*ENZYMOLOGY  *HIV-1  Infant
       Isoenzymes/*BLOOD  JOURNAL ARTICLE

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