Document 0214
 DOCN  M94A0214
 TI    A cis-acting repressive sequence that overlaps the Rev-responsive
       element of human immunodeficiency virus type 1 regulates nuclear
       retention of env mRNAs independently of known splice signals.
 DT    9412
 AU    Brighty DW; Rosenberg M; Department of Gene Expression Sciences,
       SmithKline Beecham; Pharmaceuticals, King of Prussia, PA 19406-0939.
 SO    Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8314-8. Unique Identifier :
       AIDSLINE MED/94359921
 AB    The Rev protein of human immunodeficiency virus type 1 (HIV-1) binds to
       an RNA structure, the Rev-responsive element (RRE), to enhance
       expression of the viral structural genes by relieving the nuclear
       sequestration of incompletely spliced viral transcripts. It has been
       suggested that nuclear retention of these mRNAs, in mammalian cells, is
       due to the activity of either cis-acting repressive sequence elements or
       to inefficient splicing signals. Expression of the HIV-1 envelope gene
       in transfected Drosophila cells is also dependent upon Rev coexpression
       and, hence, the mechanism of nuclear retention and Rev regulation are
       highly conserved. Here we use the Drosophila system to identify a major
       cis-acting repressive sequence element that overlaps the RRE and is
       responsible for the nuclear entrapment and Rev-dependent expression of
       HIV-1 env mRNAs. Moreover, the splice signals spanning env are not
       required for nuclear retention or Rev-dependent trans-activation of env
       mRNAs. We suggest that the RRE and its associated RNA structure are
       necessary for both the repressive and known trans-activation effects of
       Rev regulation.
 DE    Animal  Base Sequence  Cell Compartmentation  Cell Line  Cell
       Nucleus/METABOLISM  Drosophila melanogaster  DNA Primers/CHEMISTRY
       *Gene Expression Regulation, Viral  Gene Products, env/*METABOLISM
       *Genes, env  HIV-1/*GENETICS  Molecular Sequence Data  Protein
       Precursors/*METABOLISM  RNA Splicing  RNA, Messenger/GENETICS  Support,
       U.S. Gov't, P.H.S.  Transfection  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).