Document 0261
 DOCN  M94A0261
 TI    Multicenter evaluation of quantification methods for plasma human
       immunodeficiency virus type 1 RNA.
 DT    9412
 AU    Lin HJ; Myers LE; Yen-Lieberman B; Hollinger FB; Henrard D; Hooper CJ;
       Kokka R; Kwok S; Rasheed S; Vahey M; et al; Division of Molecular
       Virology, Baylor College of Medicine,; Houston, Texas.
 SO    J Infect Dis. 1994 Sep;170(3):553-62. Unique Identifier : AIDSLINE
       MED/94358487
 AB    Six procedures for quantifying plasma human immunodeficiency virus type
       1 (HIV-1) RNA were evaluated by nine laboratories. The procedures
       differed in their sample volume and preparation of samples and methods
       of amplification and detection. Coded samples in a 10-fold dilution
       series of HIV-1-spiked plasma were correctly ranked by all six
       procedures. Subsequently, coded duplicate plasma samples from 16
       HIV-1-infected patients were tested using a common set of standards.
       Several HIV-1 RNA procedures were sufficiently reproducible so that an
       empiric 4-fold change could be viewed as significant. HIV-1 RNA levels
       in the patients (up to 370,000 RNA copies/mL) correlated with proviral
       HIV-1 DNA and were inversely correlated with CD4 cell counts; HIV-1 RNA
       assays were more sensitive than plasma viremia, standard p24 antigen, or
       immune complex-dissociated p24 antigen assays. This study demonstrated
       that several HIV-1 RNA quantitative assays are ready for use in clinical
       trials.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/*DIAGNOSIS  Blood Specimen
       Collection  Comparative Study  Human  HIV Core Protein p24/BLOOD  *HIV
       Seronegativity  HIV Seropositivity/BLOOD/*DIAGNOSIS  HIV-1/*ISOLATION &
       PURIF  Laboratories/STANDARDS  Leukocyte Count  Predictive Value of
       Tests  RNA, Viral/*BLOOD  Sensitivity and Specificity  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  T4 Lymphocytes  Viremia/BLOOD
       JOURNAL ARTICLE  MULTICENTER STUDY

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).