Document 0296
 DOCN  M94A0296
 TI    A functional interaction between Rev and yeast pre-mRNA is related to
       splicing complex formation.
 DT    9412
 AU    Stutz F; Rosbash M; Howard Hughes Medical Institute, Department of
       Biology, Brandeis; University, Waltham, MA 02254.
 SO    EMBO J. 1994 Sep 1;13(17):4096-104. Unique Identifier : AIDSLINE
       MED/94357183
 AB    The HIV-1 Rev protein regulates viral gene expression by enhancing the
       export of partially spliced or nonspliced viral transcripts to the
       cytoplasm. This activity is mediated by the interaction of Rev with a
       highly structured viral RNA sequence, the Rev response element (RRE). In
       this paper, Rev regulation has been recapitulated in the yeast
       Saccharomyces cerevisiae. The data show that Rev enhances yeast pre-mRNA
       translation in an RRE-dependent manner and requires intact RNA binding
       and effector domains. In addition, reporter gene mutations in the 5'
       splice site and in the branch point are epistatic to and eliminate Rev
       responsiveness, i.e. some spliceosome assembly pathway steps precede a
       functional interaction with Rev. The results indicate that some of the
       Rev-interacting components are conserved between yeast and mammals and
       suggest that Rev acts on yeast pre-mRNA in the context of the
       spliceosome.
 DE    Biological Transport  Cell Compartmentation  DNA Mutational Analysis
       Gene Products, rev/GENETICS/*METABOLISM  Genes, Reporter
       HIV-1/*GENETICS  Metallothionein/GENETICS/METABOLISM  Models, Genetic
       Protein Binding  Recombinant Fusion Proteins/METABOLISM  RNA
       Precursors/*METABOLISM  *RNA Splicing  Saccharomyces
       cerevisiae/GENETICS/*METABOLISM  Support, U.S. Gov't, P.H.S.
       Translation, Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).