Document 0305
 DOCN  M94A0305
 TI    Prolongation of rat cardiac allograft survival by a monoclonal antibody:
       anti-rat intercellular adhesion molecule-1.
 DT    9412
 AU    Kobayashi H; Miyano T; Yamataka A; Yamataka T; Yagita H; Eto H; Ligo Y;
       Tansyo S; Kojima Y; Tamatani T; et al; Department of Paediatric Surgery,
       Juntendo University School of; Medicine, Tokyo, Japan.
 SO    Cardiovasc Surg. 1993 Oct;1(5):577-82. Unique Identifier : AIDSLINE
       MED/94356568
 AB    A mouse monoclonal antibody 1A29, which binds to the rat intercellular
       adhesion molecule-1 (ICAM-1), was studied for its effect on cardiac
       allograft survival. Expression of ICAM-1 was detectable only on vascular
       endothelium in normal heart, but was induced on myocardium associated
       with interstitial mononuclear cell infiltration during acute rejection.
       Treatment with monoclonal antibody 1A29 for 10 days after
       transplantation in 15 rats significantly prolonged allograft survival
       (mean(s.d.) 18(2) days; P < 0.001), as compared with 15 isotype-matched
       control monoclonal antibody (mean(s.d.) 10(1) days) recipients. Five-day
       treatment with monoclonal antibody 1A29, when started at 5 days after
       transplantation (the time for which acute rejection is ongoing), also
       significantly extended the survival (mean(s.d.) 12(1) days; P < 0.01) in
       seven rats. On histological examination, treatment with monoclonal
       antibody 1A29 reduced the degree of T-cell infiltration of both CD4+ and
       CD8+ subsets, and greatly reduced myocardial necrosis, vascular injury
       and intravenous thrombi. These results indicate that an anti-ICAM-1
       monoclonal antibody can be used to prevent or treat acute rejection in
       the rat cardiac allograft model and suggest that human ICAM-1 is an
       important target for immunosuppression in clinical organ
       transplantation.
 DE    Animal  Antibodies, Monoclonal/IMMUNOLOGY/*PHARMACOLOGY  Cell Adhesion
       Molecules/*IMMUNOLOGY  CD4-CD8 Ratio  Dose-Response Relationship, Drug
       Drug Administration Schedule  Graft
       Rejection/*IMMUNOLOGY/PATHOLOGY/PREVENTION & CONTROL  Graft
       Survival/*IMMUNOLOGY  Heart Transplantation/*IMMUNOLOGY/PATHOLOGY
       Leukocyte Count  Male  Mice  Mice, Inbred BALB C  Myocardium/PATHOLOGY
       Rats  Rats, Inbred F344  Rats, Inbred Lew  T-Lymphocytes/IMMUNOLOGY
       Transplantation, Homologous  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).