Document 0349
 DOCN  M94A0349
 TI    Transfer of HIV-1 to human tonsillar stromal cells following
       cocultivation with infected lymphocytes.
 DT    9412
 AU    Degrassi A; De Maria A; Lisignoli G; Zini N; Sabatelli P; Cirillo L;
       Monaco MC; Hilbert DM; Ambesi-Impiombato FS; Maraldi NM; et al;
       Dipartimento di Patologia e Medicina Sperimentale e Clinica,; Universita
       di Udine, Italy.
 SO    AIDS Res Hum Retroviruses. 1994 Jun;10(6):675-82. Unique Identifier :
       AIDSLINE MED/94355112
 AB    The susceptibility of normal human tonsillar stromal cells (HTSCs) to
       infection by HIV-1 was assessed using transmission electron microscopy
       (TEM), immunocytochemistry, and HIV-1-specific PCR analyses. Our results
       demonstrate that HTSCs are efficiently infected following cocultivation
       with the HIV-1-infected lymphoblastoid cell line GY1. Infected stromal
       cells contain intracellular viral particles present as free virus or
       associated with phagocytic vesicles. These particles express the
       HIV-1-specific p24 antigen as assessed by immunocytochemical analyses
       using an HIV-specific anti-p24 monoclonal antibody. Moreover, PCR
       analysis of genomic DNA isolated from particle-bearing tonsillar stromal
       cells identified HIV-1-specific sequences not present in either
       uninfected stromal cells or parental GY1 uninfected cells. The mechanism
       by which HIV-1 infects HTSCs does not appear to be CD4 mediated, as none
       of the human tonsillar stromal cell lines express CD4 as assessed by
       flow cytometry, immunohistochemistry, and PCR analysis. Taken together,
       these results demonstrate that human tonsillar stromal cells can be
       infected by HIV-1, and that subsequent to infection the viral genome is
       reverse transcribed, and integrated into the stromal cell DNA. The
       infection of HTSCs may contribute to HIV-1-mediated pathogenesis
       indirectly as a viral reservoir or directly by structural and functional
       modification of the lymphoid microenvironment.
 DE    Antibodies, Monoclonal  B-Lymphocytes/*MICROBIOLOGY/ULTRASTRUCTURE  Cell
       Line  Genes, gag  Human  HIV Infections/*TRANSMISSION
       HIV-1/GENETICS/IMMUNOLOGY/ULTRASTRUCTURE  Polymerase Chain Reaction
       Stromal Cells/MICROBIOLOGY/ULTRASTRUCTURE  Support, Non-U.S. Gov't
       Tonsil/*MICROBIOLOGY/ULTRASTRUCTURE  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).