Document 0351
 DOCN  M94A0351
 TI    Linear epitopes of HIV-1, presented as hybrids with Escherichia coli
       beta-galactosidase or synthetic peptides.
 DT    9412
 AU    Isaguliants MG; Sukhanova LL; Levi M; Bobkov AP; Kalinina TI; Ruden U;
       Smirnov VD; Wahren B; D.I. Ivanovsky Institute of Virology, Academy of
       Medical; Sciences, Moscow, Russia.
 SO    AIDS Res Hum Retroviruses. 1994 Jun;10(6):655-64. Unique Identifier :
       AIDSLINE MED/94355110
 AB    HIV-1 B cell epitopes from gp41, the T cell epitope of p34pol, and a
       cluster of B and T epitopes from p17gag were selected. The epitopes were
       presented as synthetic peptides and as either N- or C-terminal
       insertions into beta-galactosidase. Hybrids were efficiently expressed
       in E. coli and easily purified when epitopes were inserted at the
       beta-galactosidase C terminus. Sera from HIV-1-infected individuals
       reacted in peptide- and hybrid protein-based enzyme-linked immunosorbent
       assays (ELISAs) mostly with the immunodominant site of gp41. The second
       site of gp41 and also sites from p17 and p34 appeared to be
       immunorecessive. A few of the HIV-1-positive sera exhibited several
       immunorecessive reactivities. HIV-1-positive sera from the former Soviet
       Union and Cuba had reactivities similar to those of American, African,
       and west European sera. Some sera could not be evaluated as specifically
       HIV-1 seropositive because of their broad reactivities with a multitude
       of peptides and proteins, unrelated to HIV-1. Extensive tests were
       performed to define unspecific reactivities by absorption, blocking, and
       sandwich ELISAs. The application of the hybrid protein assay
       substantially improved the specificity of the ELISA tests. Thus, hybrid
       protein-based ELISAs appeared to be more suitable than peptide-based
       ELISAs, especially for the evaluation of immunorecessive reactivities.
 DE    beta-Galactosidase/*IMMUNOLOGY  Amino Acid Sequence  Antigenic
       Determinants/*ANALYSIS  Base Sequence  Enzyme-Linked Immunosorbent Assay
       Escherichia coli/ENZYMOLOGY/*GENETICS  Human
       HIV-1/ENZYMOLOGY/*IMMUNOLOGY  Membrane Glycoproteins/*ANALYSIS
       Molecular Sequence Data  Oligonucleotides  Protein Hybridization
       Recombinant Proteins  Support, Non-U.S. Gov't  Vaccines,
       Synthetic/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).