Document 0727
 DOCN  M94A0727
 TI    Induced expression of HIV-1 genomic RNA in latently infected cells
       followed tat, but preceded rev, mRNA expression.
 DT    9412
 AU    Peng H; Haase AT; National Centre for HIV Virology Research, Institute
       of Medical; and Veterinary Science, Adelaide, South Australia.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:109 (poster no. 68).
       Unique Identifier : AIDSLINE ASHM5/94348928
 AB    The kinetics of individual viral RNA synthesis of HIV-1 regulatory and
       structural genes in ACH2 cells after PMA induction were analysed at the
       single cell level using in situ hybridisation with splice-junction
       probes. Before PMA stimulation, only a small fraction (< 23%) of the
       cells expressed tat, nef, env or gag/genomic RNA, no cells expressed
       rev. One hour after induction, the percentage of tat, nef, env and
       gag/genomic RNA expressing cells increased to 85%, 99%, 49% and 74%,
       respectively, only 12% of the cells expressed rev mRNA. Seven hours
       after induction, 96% of the cells expressed tat mRNA; 100% expressed env
       and gag/genomic RNA; 38% expressed rev mRNA. Thereafter the expression
       level of env and gag/genomic RNA, but not tat and rev mRNA, on
       individual cells continued to increase. Our results showed that in the
       PMA-ACH-2 system, induced expression of HIV-I env and gag/genomic RNA
       followed the enhanced expression of tat and nef mRNA but preceded the
       induced expression of rev mRNA.
 DE    Cell Line  Gene Expression Regulation, Viral/PHYSIOLOGY  Genes,
       env/GENETICS  Genes, gag/GENETICS  Genes, rev/*GENETICS  Genes,
       tat/*GENETICS  Human  HIV-1/*GENETICS  RNA, Messenger/*GENETICS  RNA,
       Viral/*GENETICS  Virus Latency/*GENETICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).