Document 0804
 DOCN  M94B0804
 TI    Requirement of the human immunodeficiency virus type 1 env gene sequence
       for TAR-independent trans activation by Tat from the major
       immediate-early promoter of murine cytomegalovirus.
 DT    9412
 AU    Kim YS; McArdle Laboratory for Cancer Research, University of
       Wisconsin,; Madison, 53706.
 SO    Biochem Biophys Res Commun. 1994 Sep 15;203(2):1152-9. Unique Identifier
       : AIDSLINE MED/94380023
 AB    Previously it has been demonstrated that the human immunodeficiency
       virus type 1 (HIV-1) Tat protein mediates induction of the HIV-1 env
       expression through a TAR-independent manner in heterologous and
       homologous promoter systems (Kim and Risser, 1993, J. Virol. 67, 239;
       Kim and Panganiban, 1993, J. Virol. 67, 3739). To further explore the
       transactivation of HIV-1 env gene, I examined expression of the env, the
       bacterial CAT, and the firefly luciferase genes from a heterologous
       promoter, the major immediate-early promoter (MIEP) of murine
       cytomegalovirus (MCMV). Here we show that Tat augments gene expression
       from the MCMV MIEP only when linked to the env gene. Surprisingly, in
       contrast to the expression from an HIV-1 LTR lacking the TAR element,
       TAR-independent transactivation of env gene expression from MCMV MIEP
       did not require the full length Tat protein. In addition, deletion of
       the previously identified cis-acting Tat-responsive element in env did
       not affect Tat transactivation of the env gene expression. Thus, there
       are multiple distinct elements that mediate Tat responsiveness in the
       absence TAR.
 DE    Bacterial Proteins/PHARMACOLOGY  Base Sequence
       Cytomegalovirus/*GENETICS  Gene Expression  Gene Products,
       tat/*PHARMACOLOGY  *Genes, env  Hela Cells  Human  HIV-1/*GENETICS
       Immediate-Early Proteins/*GENETICS  Membrane Proteins/PHARMACOLOGY
       Molecular Sequence Data  *Promoter Regions (Genetics)  Repetitive
       Sequences, Nucleic Acid  Support, U.S. Gov't, P.H.S.  *Trans-Activation
       (Genetics)  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).