Document 2418
 DOCN  M94A2418
 TI    Diagnosis of HIV infection status in HIV-exposed infants.
 DT    9412
 AU    Paul M; Tetall S; Abrams E; Wang X; Bakshl S; Lesser M; Pahwa S; North
       Shore University Hospital-Cornell University Medical; College, New York
       11030.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):309 (abstract no. PC0167). Unique
       Identifier : AIDSLINE ICA10/94370157
 AB    OBJECTIVE: Early determination of HIV infection status (infected and not
       infected) in infants born to HIV-seropositive mothers. METHODS:
       Application of 4 diagnostic rules based on either single or combination
       conventional laboratory assays (HIV culture of PBMC, DNA PCR, and serum
       HIV p24 antigen) of sequential blood samples taken from 183 such
       infants. Clinical findings and serology were utilized as endpoints:
       Infected, AIDS defining illness or seropositivity > or = 15 months; n =
       51; Uninfected, seroreversion, any age, n = 132. RESULTS: The most
       useful algorithm defined by age 3 mo., with 90% sensitivity and
       specificity, an infant as infected (2 consecutive PCR positive specimens
       with the 2nd specimen also positive by culture), and uninfected (2
       consecutive negative PCR results). Consecutive positive PCR tests were
       by themselves not specific. DISCUSSION AND CONCLUSIONS: In HIV- exposed
       infants, infection status can be efficiently determined by age 3 mo.,
       utilizing a combination of PCR and culture assay results. Immunologic
       assessment and virus load estimation to correlate with disease
       progression are ongoing.
 DE    Female  Human  HIV/ISOLATION & PURIF  HIV Core Protein p24/ANALYSIS  HIV
       Infections/*DIAGNOSIS/TRANSMISSION  *HIV Seropositivity  Infant  Infant,
       Newborn  Polymerase Chain Reaction  Pregnancy  *Pregnancy Complications,
       Infectious  Sensitivity and Specificity  MEETING ABSTRACT

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