Document 2424
 DOCN  M94A2424
 TI    Susceptibility of human fetal cells to HIV infection, in vitro and in
       vivo in the SCID-HU model.
 DT    9412
 AU    Touraine JL; Sanhadji K; Firouzi R; Transplantation & Clinical
       Immunology Unit, INSERM U80, Hop. E.; Herriot, Lyon, France.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):307 (abstract no. PC0157). Unique
       Identifier : AIDSLINE ICA10/94370151
 AB    OBJECTIVE: Pre and perinatal HIV transmission from mother to child
       occurs in 17% of cases in Europe and North America, 28% in Africa.
       Susceptibility of human fetal cells to HIV infection has been studied to
       help develop a preventive strategy. METHODS: Human tissues have been
       collected from non-infected fetuses of various ages, immediately after
       death. Liver, thymus, spleen and blood cells have been incubated with
       HIV1 or HIV2 and their infection checked by measure of RT activity, p24
       release, DNA and RNA PCR, co-culture with normal CD4+ lymphocytes.
       Similar experiments were carried out in SCID-hu mice in vivo. RESULTS:
       At 9 weeks postfertilization, all fetal cells were resistant to HIV
       infection in our experimental conditions. After 11 weeks, they could be
       infected by HIV1 or HIV2. Identical cells transplanted into SCID mice to
       construct a SCID-hu model rendered the animals susceptible to HIV
       (infection of human cells present in these mice, in more than 90% of
       inoculated animals, whether HIV was injected in the graft itself or
       intravenously). DISCUSSION AND CONCLUSIONS: Human fetuses can be
       infected with HIV1 or HIV2 as soon as the end of the first trimester of
       gestation. Due to placenta protection, the infection actually occurs
       seldom before the end of the second trimester. In vitro and in vivo
       models are now ready for analysis of potential prophylaxis using
       specific antibodies or chemotherapies.
 DE    Animal  Cell Transplantation  Cells, Cultured  Female
       Fetus/*MICROBIOLOGY  Human  HIV
       Infections/CONGENITAL/MICROBIOLOGY/PREVENTION & CONTROL  HIV-1/*GROWTH &
       DEVELOPMENT  HIV-2/*GROWTH & DEVELOPMENT  Infant, Newborn  Mice  Mice,
       SCID  Pregnancy  Pregnancy Complications, Infectious/MICROBIOLOGY
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).