Document 2430
 DOCN  M94A2430
 TI    Risk factors for vertical transmission of HIV-1.
 DT    9412
 AU    Ometto L; De Rossi A; Zanotto C; Maccabruni A; Caselli D; Chieco-Bianchi
       L; Inst. Oncology, University of Padova, Italy.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):306 (abstract no. PC0155). Unique
       Identifier : AIDSLINE ICA10/94370145
 AB    OBJECTIVE: To define risk factors for vertical transmission of HIV-1.
       STUDY POPULATION: Fifty-two HIV-1 infected mothers. Mother-child pair
       samples were obtained at time of delivery. METHODS: HIV-1 copy number in
       patient PBMC (Peripheral Blood Mononuclear Cells) was determined by
       quantitative DNA-PCR. Plasma samples were tested for HIV-1 p24 free
       antigen, p24 immunocomplexed antigen and antibodies to the V3 loop,
       using Elisa assays. The biological phenotype of viral isolates from
       mothers was defined according to cell tropism and syncytium inducing
       capability by viral culture in primary lymphocytes and macrophages, and
       in MT-2 cells. RESULTS: Of 52 mothers enrolled in this study, 6 mothers
       transmitted HIV infection to their child. We did not find a correlation
       between CD4+ count, p24 antigenemia, presence of antibodies to the V3
       loop, viral burden in PBMC, and mother to child transmission of HIV-1.
       Of particular relevance, we found that viral isolates from all
       transmitting mothers macrophage-tropic or macrophage-lympho-amphotropic,
       while isolates from the majority of non-transmitting mothers did not
       show this pattern. CONCLUSIONS: These findings strongly suggest that
       monocytotropism of the maternal viral isolate is critical for vertical
       transmission of HIV-1.
 DE    Female  Human  HIV Antigens/ANALYSIS  HIV
       Infections/IMMUNOLOGY/MICROBIOLOGY/*TRANSMISSION  *HIV-1/IMMUNOLOGY
       Infant, Newborn  Leukocyte Count  Pregnancy  *Pregnancy Complications,
       Infectious/IMMUNOLOGY/MICROBIOLOGY  Risk Factors  T4 Lymphocytes
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).