Document 2503
 DOCN  M94A2503
 TI    Cytokine(s) released by CD8+ T cells from HIV-seronegative individuals
       suppressing in vitro HIV replication.
 DT    9412
 AU    Tsuchie H; Tesfamariam N; Trickett A; Lam-Po-Tang PR; Dwyer JM; Ichimura
       H; Kurimura T; Research Institute for Microbial Diseases, Osaka
       University,; Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):29 (abstract no. 088A). Unique
       Identifier : AIDSLINE ICA10/94370072
 AB    OBJECTIVE: To study the effect of CD8+ T lymphocytes from
       HIV-seronegative individuals on in vitro HIV replication in naturally
       infected CD4+ T cells. METHODS: By using immunomagnetic beads, CD4+ and
       CD8+ T cells were positively selected from peripheral blood mononuclear
       cells of HIV-seronegative or HIV-seropositive individuals. The CD4+ T
       cells from HIV-seropositive individuals were mixed with CD8+ T cells
       from HIV-seropositive or HIV-seronegative individuals at a ratio of 1:3
       and cocultured with or without using a semipermeable membrane in
       transwell devices. The culture supernatants were assayed for HIV-1 p24
       antigen. RESULTS: When autologous CD8+ T cells were added to CD4+ T
       cells from HIV-seropositive individuals, HIV-1 antigen production was
       prevented, even if separated by the membrane. CD8+ T cells from most of
       HIV-seronegative individuals suppressed HIV replication if cocultured.
       In case of the separated coculture, CD8+ T cells from some of
       HIV-seronegative individuals could prevented or sharply reduced the HIV
       replication. CONCLUSION: CD8+ T cells from HIV-seronegative individuals
       could release cytokine(s) suppressing in vitro HIV replication in
       naturally infected CD4+ T cells.
 DE    *Antigens, CD8  Cells, Cultured  Human  HIV Core Protein
       p24/BIOSYNTHESIS/ISOLATION & PURIF  HIV Seronegativity/*IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  T-Lymphocyte Subsets/*IMMUNOLOGY  T4
       Lymphocytes/MICROBIOLOGY  *Virus Replication  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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