Document 2587
 DOCN  M94A2587
 TI    In vitro and in vivo activities of sparfloxacin against Chlamydia
       trachomatis.
 DT    9412
 AU    Nakata K; Matsui T; Miyazaki S; Arakawa S; Ishigami J; Kamidono S; Kobe
       University, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):270 (abstract no. PB0509). Unique
       Identifier : AIDSLINE ICA10/94369988
 AB    OBJECTIVE: To test the in vitro and in vivo activity of sparfloxacin,
       levofloxacin, tosufloxacin, clarithromycin and minocycline against C.
       trachomatis. METHODS: The susceptibility of C. trachomatis (25 strains)
       to sparfloxacin and the reference drugs was tested in vitro by
       inhibiting inclusion body formations in McCoy cells. The vivo
       antichlamydial activities of sparfloxacin and the reference drugs on C.
       trachomatis KN infection were examined in experimental leukopenic mice.
       Leukopenic mice were inoculated transurethrally with C. trachomatis, and
       the drugs were given then orally twice a day for 3 days. On day 5 of
       postinfection, the urinary bladder was removed and the mucous bladder
       membranes were rubbed off with swabs which were then cultured with McCoy
       cells. The absence of organisms in the urinary bladder was the sole
       endpoint of therapeutic success. RESULTS: The MICs (microgram/ml) of
       sparfloxacin, levofloxacin, tosufloxacin, clarithromycin, and
       minocycline against 25 clinical isolates were 0.025-0.05, 0.25-0.5,
       0.063-0.125, 0.025-0.05, and 0.025-0.05, respectively. The ED50s (mg/kg)
       of sparfloxacin, levofloxacin, tosufloxacin, clarithromycin, and
       minocycline on urinary tract infection with C. trachomatis KN were 3.4,
       13.8, 13.6, 15.6, and 4.2, respectively. CONCLUSIONS: Based on results,
       sparfloxacin is expected to be useful for treatment of C. trachomatis
       infection in humans.
 DE    Animal  Anti-Infective Agents, Fluoroquinolone/*PHARMACOLOGY  Chlamydia
       trachomatis/*DRUG EFFECTS  Clarithromycin/PHARMACOLOGY  Comparative
       Study  Dose-Response Relationship, Drug  Mice  Microbial Sensitivity
       Tests  Minocycline/PHARMACOLOGY  Naphthyridines/PHARMACOLOGY
       Quinolones/*PHARMACOLOGY  MEETING ABSTRACT

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