Document 2590
 DOCN  M94A2590
 TI    Anal infection with multiple HPV types in men with symptomatic HIV
       disease.
 DT    9412
 AU    Palefsky JM; Holly EA; Ahn D; Department of Laboratory Medicine,
       University of California, San; Francisco 94143.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):27 (abstract no. 081B). Unique
       Identifier : AIDSLINE ICA10/94369985
 AB    OBJECTIVE: The incidence of anal cancer is increasing in association
       with the AIDS epidemic. This disease is associated with human
       papillomavirus (HPV) infection. However, little is known about anal HPV
       infection in men with advanced HIV disease. In this study, we
       characterized changes in HPV type and level over 1 year in this
       population. METHODS: HPV testing was performed in 57 homosexual men with
       symptomatic HIV disease at baseline, and at 6 and 12 months using 2
       methods. L1 consensus primer PCR was used to detect low level or high
       level infection with HPV types 6, 11, 16, 18, 31, 33, 35, 39, 45, 51,
       and 52. Hybrid Capture (HC) was used to detect high level infection
       (indicating active viral replication) with HPV groups 6/11, 42/43/44,
       16/18, 31/33/35, and 45/51/52/56, as well as HPV type 16 alone. The
       concentrations of HPV 16 and 6/11 DNA in the anal specimens were
       calculated using standard curves at 6 months into the study. RESULTS:
       The prevalence of PCR positivity among the men increased from 67% to 84%
       after one year. At baseline, multiple types were found in 56% of men
       with a mean of 1.9 types. After one year, this rose to 81% with a mean
       of 3.1 types. The prevalence of HC positivity increased from 54% to 77%
       after one year. At baseline, multiple groups were found in 32% of men
       with a mean of 1.1 groups. After one year, this rose to 50% with a mean
       of 1.8 groups. Among those with HPV 16 or 6/11 by HC at 6 months, the
       median HPV 16 level was 125 pg/ml (n = 10, range 11-2080), and 6/11 was
       912 pg/ml (n = 7, range 28-2860). CONCLUSIONS: HPV infection persists in
       this population, including low level infection. The proportion of men
       with multiple types is high and continues to increase over time. The
       concentrations of different HPV types are also very high but vary widely
       and there is no clear selective advantage of one group over the other.
 DE    Anus Diseases/COMPLICATIONS/*MICROBIOLOGY  Homosexuality  Human  HIV
       Infections/*COMPLICATIONS  Male  Papillomavirus,
       Human/CLASSIFICATION/*ISOLATION & PURIF  Papovaviridae
       Infections/COMPLICATIONS/*MICROBIOLOGY  Polymerase Chain Reaction
       Serotyping  Tumor Virus Infections/COMPLICATIONS/*MICROBIOLOGY  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).