Document 2654
 DOCN  M94A2654
 TI    Efficacy of HIV antigen acid dissociation in pediatric AIDS.
 DT    9412
 AU    Canizal A; Zarwanitzer S; Benetucci J; Laube G; Rubinstein E; Sosa CE;
       Laboratorio de Retrovirus, FUNDAI y Pediatria, Buenos Aires.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):255 (abstract no. PB0448). Unique
       Identifier : AIDSLINE ICA10/94369921
 AB    OBJETIVES: To asses the efficacy of acid pretreatment in increasing
       sensitivity of p24 Antigen detection. METHODS: Forty four sera from
       children born to seropositive mothers were investigated with or without
       acid pretreatment and compared to HIV co-culture as a standard. We used
       CIH (pH 2-3) to disrupt immunocomplexes. Samples were incubated for one
       hour followed by neutralization with NaOH. EIA was performed and
       positive specimens were confirmed by a commercial kit (Organon,
       Teknika). RESULTS: Age of children ranged from 1 to 24 months (mean
       7.2). Clinical status: P0 n = 27; P1 n = 1; P2 n = 16. Twelve children
       from whom pretreated antigen was detected were asymptomatic (P0 status)
       and below six months of age. Noteworthy, pretreated antigen was detected
       in four samples which were negative co-culture. TABULAR DATA, SEE
       ABSTRACT VOLUME. CONCLUSIONS: Acid pretreatment increased the
       sensitivity of the assay from 40.9% to 72.7%. This procedure showed to
       be very valuable for early diagnosis of HIV infected children, specially
       for developing countries.
 DE    Acquired Immunodeficiency Syndrome/*DIAGNOSIS/IMMUNOLOGY/  TRANSMISSION
       Antigen-Antibody Complex/*IMMUNOLOGY  *AIDS Serodiagnosis  Female
       Follow-Up Studies  Human  HIV Core Protein p24/*BLOOD  Immunoenzyme
       Techniques  Infant  Infant, Newborn  Predictive Value of Tests
       Pregnancy  MEETING ABSTRACT

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