Document 2664
 DOCN  M94A2664
 TI    Testing pooled sera for HIV antibodies.
 DT    9412
 AU    Torimiro JN; Ashu FA; Lobe VE; Ndumbe PM; Center for the Study and
       Control of Communicable Diseases,; F.M.B.S., University of Yaounde I,
       Cameroon.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):252 (abstract no. PB0436). Unique
       Identifier : AIDSLINE ICA10/94369911
 AB    OBJECTIVES: To assess whether the testing of pooled sera is technically
       feasible, cost-effective and accurate for estimating seroprevalence in
       large population surveys. METHODS: 760 sera of known HIV reactivity were
       made into 152 pools by mixing 100 microliters of each constituent
       sample, (1 in 5) and 76 pools by mixing 100 microliters of 2 pools of 5
       (1 in 10). Testing was with 3 ELISAs (Vironostika, Enzygnost, Elavia)
       and 2 rapid tests (PATH Dipstick and Clonatec). RESULTS: TABULAR DATA,
       SEE ABSTRACT VOLUME. DISCUSSION AND CONCLUSIONS: Pooling at 1 in 5 is
       more sensitive and specific than for 1 in 10 pools. An overall gain in
       time (about 48%) and cost savings are obtained with such schemes.
       Testing pooled sera ought to be widely used in seroepidemiologic studies
       in developing countries.
 DE    AIDS Serodiagnosis/*METHODS  *Blood Banks  Cameroon/EPIDEMIOLOGY
       Comparative Study  Enzyme-Linked Immunosorbent Assay/*METHODS  Human
       HIV Antibodies/*BLOOD  HIV
       Seropositivity/DIAGNOSIS/*EPIDEMIOLOGY/IMMUNOLOGY/  TRANSMISSION  *HIV
       Seroprevalence  *Population Surveillance  Predictive Value of Tests
       MEETING ABSTRACT

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