Document 2666
 DOCN  M94A2666
 TI    HIV1/HIV2 EIA seronegativity in HIV1 subtype O infected patients in
       France.
 DT    9412
 AU    Ly TD; Lousset-Ajaka I; Chaix ML; Saragosti S; Brun-Vesinet F; Simon F;
       Institut Alfred Fournier, Paris, France.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):252 (abstract no. PB0437). Unique
       Identifier : AIDSLINE ICA10/94369909
 AB    OBJECTIVE: To study the serological reactivity of samples collected from
       7 HIV1 subtype O infected patients PATIENTS-METHOD: 7 patients
       (CDCII-III n = 3, CDC IV n = 4) originally from Cameroon were
       hospitalised in Paris, France. HIV1 subtype O infection was diagnosed by
       using PCR specific subtype O primers and serological reactivity on
       specific Env subtype peptides. Sequential samples were tested on
       different HIV1-2 EIA and rapid tests based on different antigens and
       conjugate principles. RESULTS: Discordant results were observed
       according to the antigen or to the conjugate used in the test. All the
       samples were strongly reactive in EIA based on whole virus or
       recombinant antigens with indirect conjugate fixation as shown in the
       table. CONCLUSION: Screening test for HIV antibodies based on Env
       peptides or the sandwich method could lack sensitivity in the case of
       HIV1 subtype O infection. TABULAR DATA, SEE ABSTRACT VOLUME.
 DE    *AIDS Serodiagnosis  Cameroon/ETHNOLOGY  Comparative Study  France
       Human  HIV Antibodies/*BLOOD  HIV
       Seropositivity/CLASSIFICATION/*DIAGNOSIS/IMMUNOLOGY
       HIV-1/CLASSIFICATION/*IMMUNOLOGY  HIV-2/*IMMUNOLOGY  *Immunoenzyme
       Techniques  Predictive Value of Tests  Sensitivity and Specificity
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).