Document 2674
 DOCN  M94A2674
 TI    Interaction of HIV-1 proteins with antibodies on the surface of latex
       particles.
 DT    9412
 AU    Manykin AA; Krylov AS; Efremova EV; Institute of Virology, Moscow,
       Russia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):250 (abstract no. PB0428). Unique
       Identifier : AIDSLINE ICA10/94369901
 AB    OBJECTIVE: To visualize HIV-1 protein-antibody interactions and to study
       its distributions on the surface of latex particles. METHODS:
       Polysterene latex microspheres (D-08 micron) with adsorbed rabbit
       antibodies against HIV-1 are used and visualizations of antibodies are
       carried out by immuno-EM technique. RESULTS: Latex microspheres are
       mixed with solutions with different concentrations of virus antigen and
       several minutes after agglutination starts, microspheres are placed on
       EM-grids and proceeded with gold antirabbit conjudate. Different degrees
       of conjugate - antibody interaction and antigenes distributions on the
       surface of microspheres are observed. DISCUSSION AND CONCLUSIONS: It is
       supposed uneven conjugate-antibody distributions depend on properties of
       latex surface.
 DE    Antigen-Antibody Reactions/*IMMUNOLOGY  AIDS Serodiagnosis/*METHODS
       Human  HIV Antibodies/*BLOOD  HIV Antigens/IMMUNOLOGY  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Latex Fixation
       Tests/*METHODS  Microspheres  Predictive Value of Tests  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).