Document 2680
 DOCN  M94A2680
 TI    Lack of hundred percent sensitivity of ELISA in Indian patients with
       AIDS.
 DT    9412
 AU    Sehgal S; Immunopathology Department, P.G.I.M.E.R., Chandigarh.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):249 (abstract no. PB0425). Unique
       Identifier : AIDSLINE ICA10/94369895
 AB    At the Postgraduate Medical Institute, Chandigarh, India, a Surveillance
       Centre was set up in 1987 to monitor time trends and risk factors in HIV
       infection. By December, 1993, 91384 cases (72190 donors and 19194 high
       risk cases) were screened by ELISA and 275 confirmed positive by Western
       blot using ARC and later W.H.O. criteria. DNA amplification was done by
       standard PCR protocols when necessary. Thirty five cases were foreign
       students mainly from an African country. Of the remaining 240 cases, 51
       had acquired infection through infected blood/products and only 2 had
       acquired infection through I.V. drug use indicating gross differences in
       risk factors in different parts of India. The high risk group showed a
       steady increase from nil to 10.8 per thousand. At least 65 cases had
       full blown disease and 40 were already dead. Interestingly two cases
       with full blown disease tested negative by one type of WHO approved
       peptide based ELISA and were positive by another kit. One case had
       cerebral toxoplasmosis and another had HIV associated polymyositis. It
       is concluded that a) there are different predominant risk factors in
       different parts of India,b) kits designated to have 100% sensitivity may
       not depict similar sensitivity in Indian patients particularly in full
       blown disease with falling antibodies rising antigenemia and possible
       genetic variants.
 DE    AIDS Serodiagnosis/*METHODS  Blotting, Western  Comparative Study
       *Developing Countries  Enzyme-Linked Immunosorbent Assay/*METHODS  Human
       HIV Antibodies/*BLOOD  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY/TRANSMISSION  India  Predictive
       Value of Tests  Reagent Kits, Diagnostic  Risk Factors  Sensitivity and
       Specificity  MEETING ABSTRACT

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