Document 2685
 DOCN  M94A2685
 TI    Presence of HIV IgM in early seroconversion and in the phase of
       infection.
 DT    9412
 AU    Hellings JA; van Binsbergen J; Trines C; Organon Teknika, Boxtel, The
       Netherlands.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):248 (abstract no. PB0422). Unique
       Identifier : AIDSLINE ICA10/94369890
 AB    OBJECTIVE: To determine the detectability of IgM antibodies to HIV-I gag
       and env protein during various stages of HCV infection by using a very
       sensitive ELISA method. METHODS: Twenty commercially available
       seroconversion series and 200 asymptomatic carrier samples were tested.
       A very sensitive HIV IgM ELISA was established using microELISA plates
       coated with recombinant HIV-1 p24, purified viral gp160 and HIV-2 gp36
       peptide (aa 592-603). Data were compared with those of second generation
       (anti-IgG) and third generation sandwich type HIV screening tests.
       RESULTS: All twenty seroconversion series were IgM positive, in some
       cases more than two weeks before IgG becomes detectable. Third
       generation sandwich type HIV screening assays (c.q. Vironostika HIV
       Uni-Form II) are as sensitive to early seroconversion as the IgM
       specific test. Surprisingly some 50% of asymptomatic carrier samples
       contained detectable amounts of IgM as well. CONCLUSION: Anti-HIV IgM is
       a consistent marker of early seroconversion and early detection of
       seroconversion, therefore requires a good sensitivity to IgM-type
       anti-HIV. Anti-HIV-IgM however, may persist during the asymptomatic
       stage of HIV infections. Future studies are aimed at a potential
       predictive value of this marker for disease progression.
 DE    AIDS Serodiagnosis/*METHODS  Biological Markers/BLOOD  Enzyme-Linked
       Immunosorbent Assay/*METHODS  Gene Products, env/IMMUNOLOGY  Human  HIV
       Antibodies/*BLOOD  HIV Antigens/IMMUNOLOGY  HIV Core Protein
       p24/IMMUNOLOGY  HIV Seropositivity/CLASSIFICATION/*DIAGNOSIS/IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  IgM/*BLOOD  Predictive Value of Tests  Protein
       Precursors/IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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