Document 2689
 DOCN  M94A2689
 TI    The correlation between HIV synthetic peptide-based ELISA sensitivity
       and peptide conformation.
 DT    9412
 AU    Nikolaeva I; Sidorovitch IG; Khaitov RM; Andreev SM; Prokopenko VD;
       Tarasova SO; Denisova SK; Institute of Immunology, Moscow, Russia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):247 (abstract no. PB0416). Unique
       Identifier : AIDSLINE ICA10/94369886
 AB    OBJECTIVE. To evaluate the comparative immunoreactivity of synthetic
       peptide (SP) fragments from immunodominant region of gp41 HIV-1 for the
       greater SP-based ELISA sensitivity. METHODS. Antigens: SP(1)aa584-604,
       Wang, 1986; (2)aa601-624, linear; (3)aa601-624, cyclic, with C603-C609
       S-S bond; Sera panels: A (29 WB confirmed HIV-1 positive samples from
       patients followed-up at the Inst.Imunol. since 1985); B (16 WB confirmed
       HIV-1 positive samples with low antiHIV antibody titers, lycensed in RF
       for quality control of commercial HIV ELISAs), C (11 WB confirmed HIV-1
       positive samples, Uganda). RESULTS. The ratios of # samples tested/#
       samples detected as positives were for panel A 23/29 (SP1), 28/29 (SP2),
       29/29 (SP3); for panel B 0/16 (SP1), 9/16 (SP2), 16/16 (SP3); for panel
       C 4/11 (SP1), 6/11 (SP2), 10/11 (SP3). DISCUSSION AND CONCLUSIONS.
       Cyclic SP3 provides more accurate HIV Ab detection, than its linear
       analog (SP3 recognizes 55 of 56 specimens tested vs 43 of 56 for SP2),
       especially for the weak (low Ab titer) sera, probably due to better
       modelling of the At-binding site of viral gp41.
 DE    AIDS Serodiagnosis/*METHODS  Blotting, Western  Comparative Study
       Enzyme-Linked Immunosorbent Assay/*METHODS  Human  HIV Antibodies/*BLOOD
       HIV Envelope Protein gp41/*IMMUNOLOGY  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Peptide
       Fragments/*IMMUNOLOGY  Predictive Value of Tests  Protein Conformation
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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