Document 2695
 DOCN  M94A2695
 TI    Automated chemiluminescence assay for the detection of antibody to human
       immunodeficiency virus type-1 (HIV) in serum and whole blood.
 DT    9412
 AU    Matsuda J; Tsukamoto M; Saitoh N; Gohchi K; Gotoh M; Kawamoto M;
       Takizawa N; Department of Medicine, Teikyo University School of
       Medicine,; Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):246 (abstract no. PB0414). Unique
       Identifier : AIDSLINE ICA10/94369880
 AB    OBJECTIVE: To evaluate a newly developed fully automated screening assay
       for anti-HIV antibody measurement employing chemiluminescence technique,
       which enabled us to obtain results within 13 minutes and to handle 150
       samples per hour. SUBJECTS AND METHODS: The subjects were 61 patients
       with HIV infection and 244 HIV negative disease controls and 100 healthy
       controls. Serum samples from all subjects as well as anti-coagulated
       whole blood from available subjects were analyzed. Anti-HIV antibody was
       measured using fully automated analyzer (JIA-FS 150, Japan Electric)
       based on chemiluminescence assay developed by us. RESULTS: None of the
       HIV-negative sera and whole blood from the healthy controls, diseased
       controls were found to be positive for HIV antibody. 61 sera and
       available whole blood of the HIV positive patients were judged positive
       for HIV-antibody. Thus the specificity and sensitivity of this test were
       revealed to be satisfactory. Furthermore, we have confirmed that samples
       extracted from filter paper absorbed dried whole blood were also
       applicable to this system. CONCLUSION: This test may be useful for mass
       screening for HIV antibody at blood banks, commercial laboratories,
       emergency departments, and intensive care units as well as for screening
       test at anonymous testing sites, and for mail-order test from remote
       areas and developing countries.
 DE    AIDS Serodiagnosis/*METHODS  *Chemiluminescence  Human  HIV
       Antibodies/*BLOOD  HIV Seronegativity/IMMUNOLOGY  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Predictive
       Value of Tests  MEETING ABSTRACT

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