Document 2696
 DOCN  M94A2696
 TI    HIV antibody responses determined by the ELISA sandwich capture assay
       using recombinant glutathione-S-transferase-fused antigens.
 DT    9412
 AU    Tachibana Y; Yamauchi Y; Sairenji T; Takahashi H; Kurata T; Kojima A;
       Department of Pathology, National Institute of Health, Tokyo,; Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):245 (abstract no. PB0410). Unique
       Identifier : AIDSLINE ICA10/94369879
 AB    OBJECTIVE: To develop an ELISA system with a low background level which
       enables to quantify the HIV-specific antibodies using E. coli-derived
       recombinant antigens. METHODS: HIV-1 gag p17, p24, and pol integrase
       were expressed as the glutathione-S-transferase (GST) fusion proteins.
       The reombinant antigens partially purified by a glutachione affinity
       chromatography were immobilized on microtiter plates which had been
       coated with the monoclonal antibody to GST (1G05). Antibody levels in a
       total of 147 sera from 36 HIV-1-infected patients and 45 healthy donors
       were determined by the improved sandwich capture ELISA. RESULTS: The
       ELISA system significantly distinguished between HIV-positive group and
       healthy group (p < 0.0001). There were significant correlations among
       the kinetics of anti-p17, p24, integrase, and CD4+ cell ratios in
       certain HIV-1-infected patients during the course of infection (p <
       0.01). In some cases, however, no significant correlations were observed
       among all antibodies. CD4+ cell ratios are not always correlate with
       kinetics of the antibody responses during our observation period.
       CONCLUSION: Our ELISA system provides a fast and relatively cheap
       diagnostic tool to determine the HIV-specific antibody titers in human
       sera.
 DE    AIDS Serodiagnosis/*METHODS  DNA Nucleotidyltransferases/IMMUNOLOGY
       Enzyme-Linked Immunosorbent Assay/*METHODS  Gene Products,
       gag/IMMUNOLOGY  *Glutathione Transferases  Human  HIV Antibodies/*BLOOD
       HIV Antigens/*IMMUNOLOGY  HIV Core Protein p24/IMMUNOLOGY  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Predictive
       Value of Tests  Recombinant Proteins/IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).