Document 2727
 DOCN  M94A2727
 TI    Confirmatory diagnosis of HIV-2 antibodies. Evaluation of a western
       blot.
 DT    9412
 AU    Cruz O; Perez MT; Lobaina L; Noa B; Diaz H; Laboratorio de
       Investigaciones del SIDA, Habana, Cuba.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):239 (abstract no. PB0384). Unique
       Identifier : AIDSLINE ICA10/94369848
 AB    OBJECTIVE: To evaluate a Western blot (WB) system for detecting HIV-2
       antibodies. METHOD: 226 serum samples were studied, 7 of them were HIV-2
       positives, 53 from blood donors, 10 positive to Chagas Disease, 1
       positive to malaria. 9 with anti DNA antibodies from patients with
       Systemic Lupus Erythematosus (SLE), and 11 with HLA antibodies. Besides,
       135 HIV-1 positive samples were evaluated, 75 of them were from Uganda
       patients and 60 from a known panel belonging to the AIDS Reference
       Laboratory. All samples were tested by the Western blot system DAVIH
       blot II following the instructions indicated by the manufacturers.
       RESULTS: All the positive samples were correctly identified by the
       DAVIH-blot II system. There were 6 indeterminates within the blood
       donors group and 2 indeterminates from the anti HLA group. The positive
       samples with the HIV-2 antigens showed a 91% of cross-reactivity being
       predominant the reactions to the p26 (70%) and the gp36 (56%).
       CONCLUSIONS: DAVIH blot II system showed to be highly sensitive in
       detecting all the positive samples to HIV-2, and the specificity
       coincided with the previously results given by HIV-1 WB in low risk
       population. On the other hand, it was not identified any HIV-1
       seropositive as reactive to HIV-2, that is why it is useful to
       distinguish HIV-1 and HIV-2 positives.
 DE    Blood Donors  *Blotting, Western  Cross Reactions/IMMUNOLOGY  Evaluation
       Studies  Human  HIV Antibodies/*BLOOD  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-2/*IMMUNOLOGY  Predictive
       Value of Tests  Uganda  MEETING ABSTRACT

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